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A New Design of Protein Crystallization Plates To Expand Concentration Screening Space in Cross-Diffusion Microbatch and Microbatch Methods

Published on Feb 3, 2016in Crystal Growth & Design4.15
· DOI :10.1021/acs.cgd.5b00702
Chen Dong3
Estimated H-index: 3
,
Chen-Yan Zhang8
Estimated H-index: 8
+ 3 AuthorsDa-Chuan Yin16
Estimated H-index: 16
Abstract
Protein crystallizes at specific conditions (correct precipitants, appropriate concentrations of protein and precipitants, suitable pH and correct temperature, etc.). If the conditions are not appropriate, crystallization will not occur. In protein crystallization screening, the target protein is mixed one by one with many chemical agents and then incubated at a set temperature. If the concentrations of the chemical agents and the target proteins are not in a range suitable for crystallization, the crystallization will not occur. To expand the concentration screening space, we propose in this paper a new design of protein crystallization plates for cross-diffusion microbatch and microbatch methods. The new plates have 96 units corresponding to the conditions of the commercial screening kits, and each unit contains four wells for holding the crystallization droplets. By dispensing crystallization droplets to the four wells at different volume ratios of protein to precipitant solutions, we can obtain four d...
  • References (34)
  • Citations (4)
References34
Newest
#1Rui-Qing Chen (NPU: Northwestern Polytechnical University)H-Index: 5
#2Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
Last.Yue Liu (NPU: Northwestern Polytechnical University)H-Index: 6
view all 6 authors...
#1Qin-Qin Lu (NPU: Northwestern Polytechnical University)H-Index: 8
#2Xu-Zhuo Xie (NPU: Northwestern Polytechnical University)H-Index: 2
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
view all 7 authors...
#1Qin-Qin Lu (NPU: Northwestern Polytechnical University)H-Index: 8
#2Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
Last.Rui-Qing Chen (NPU: Northwestern Polytechnical University)H-Index: 5
view all 5 authors...
#1Fermín Otálora (UGR: University of Granada)H-Index: 22
#2Jose A. Gavira (UGR: University of Granada)H-Index: 23
Last.Juan Manuel García-Ruiz (UGR: University of Granada)H-Index: 32
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#1Renaud Vincentelli (CNRS: Centre national de la recherche scientifique)H-Index: 22
#2Stéphane Canaan (CNRS: Centre national de la recherche scientifique)H-Index: 28
Last.Christophe Bignon (CNRS: Centre national de la recherche scientifique)H-Index: 13
view all 10 authors...
Cited By4
Newest
#1Hai Hou (NPU: Northwestern Polytechnical University)H-Index: 3
#2Miao Shi (NPU: Northwestern Polytechnical University)H-Index: 1
Last.Xudong Deng (NPU: Northwestern Polytechnical University)H-Index: 8
view all 11 authors...
#1Chen-Yan Zhang (NPU: Northwestern Polytechnical University)H-Index: 8
#2Chen Dong (NPU: Northwestern Polytechnical University)H-Index: 3
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
view all 9 authors...
#1Rui-Qing Chen (NPU: Northwestern Polytechnical University)H-Index: 5
#2Qing-Di Cheng (NPU: Northwestern Polytechnical University)H-Index: 4
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
view all 8 authors...
View next paperThe advantages of using a modified microbatch method for rapid screening of protein crystallization conditions