TET‐catalyzed oxidation of intragenic 5‐methylcytosine regulates CTCF‐dependent alternative splicing

Intragenic 5‐methylcytosine and CTCF mediate opposing effects on pre‐ mRNA splicing: CTCF promotes inclusion of weak upstream exons through RNA polymerase II pausing, whereas 5‐methylcytosine evicts CTCF , leading to exon exclusion. However, the mechanisms governing dynamic DNA methylation at CTCF ‐binding sites were unclear. Here, we reveal the methylcytosine dioxygenases TET 1 and TET 2 as active regulators of CTCF ‐mediated alternative...