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The fungicide Pristine® inhibits mitochondrial function in vitro but not flight metabolic rates in honey bees.

Published on Mar 1, 2016in Journal of Insect Physiology2.86
· DOI :10.1016/j.jinsphys.2015.12.003
Jacob B. Campbell4
Estimated H-index: 4
(ASU: Arizona State University),
Rachna Nath2
Estimated H-index: 2
(ASU: Arizona State University)
+ 3 AuthorsJon F. Harrison41
Estimated H-index: 41
(ASU: Arizona State University)
Abstract
Abstract Honey bees and other pollinators are exposed to fungicides that act by inhibiting fungal mitochondria. Here we test whether a common fungicide (Pristine®) inhibits the function of mitochondria of honeybees, and whether consumption of ecologically-realistic concentrations can cause negative effects on the mitochondria of flight muscles, or the capability for flight, as judged by CO2 emission rates and thorax temperatures during flight. Direct exposure of mitochondria to Pristine® levels above 5 ppm strongly inhibited mitochondrial oxidation rates in vitro. However, bees that consumed pollen containing Pristine® at ecologically-realistic concentrations (≈1 ppm) had normal flight CO2 emission rates and thorax temperatures. Mitochondria isolated from the flight muscles of the Pristine®-consuming bees had higher state 3 oxygen consumption rates than control bees, suggesting that possibly Pristine®-consumption caused compensatory changes in mitochondria. It is likely that the lack of a strong functional effect of Pristine®-consumption on flight performance and the in vitro function of flight muscle mitochondria results from maintenance of Pristine® levels in the flight muscles at much lower levels than occur in the food, probably due to metabolism and detoxification. As Pristine® has been shown to negatively affect feeding rates and protein digestion of honey bees, it is plausible that Pristine® consumption negatively affects gut wall function (where mitochondria may be exposed to higher concentrations of Pristine®).
  • References (38)
  • Citations (13)
References38
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#1Gloria DeGrandi-Hoffman (ARS: Agricultural Research Service)H-Index: 24
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