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Monosodium Glutamate Toxic Effect on Spleen Structure and Potentiality of Recovery in Adult Albino Rats

Published on Dec 1, 2012
· DOI :10.21608/eajbsz.2012.13513
Hala M. Ebaid4
Estimated H-index: 4
,
Hend M. Tag4
Estimated H-index: 4
Cite
Abstract
Histological effects of Monosodium glutamate (MSG) commonly used as food additive on the spleen of adult Wistar rats were carefully studied. The rats (n=30), average weight of 115 gm were randomly assigned into four treatments (n=6) and control (n=6) groups. The rats in the treatment groups received 4 mg/kg IP of MSG for fourteen days, while the control group receives the same volume of distilled water intraperitoneally. The rats were sacrificed after the last day of injection (group 1) and on 14 (group 2), 28 (group 3) and 42 day (group 4) after cessation of the treatment with MSG. The spleen was carefully dissected out and quickly fixed in 10% buffered formaldehyde for routine histological study after H&E method. The histological findings after H&E methods for group 1 indicated that the treated sections of the spleen showed atrophy of the while pulp, germinate centers were missing and red pulp of the spleen showed aplasia. The treatment (Group 2) showed vacuolation of some splenic cells with decreased cellularity, and sinusoidal spaces were large. Animals of group 3 showed slight atrophy in the white pulp and aplasia of red pulp where it began to retain normal structure. In the animals sacrificed at the end of the experimental period, the spleen retained its normal structure. These findings indicate that MSG consumption may have some deleterious effects on the spleen of adult Wistar rats which is reversible and does not lead to permanent damage but the normal structure of the spleen would need a long time to be regained. It is recommended that further studies aimed at corroborating these findings be carried out.
  • References (20)
  • Citations (4)
Cite
References20
Newest
Published on Aug 1, 2010in Nature Medicine30.64
Francesca Fallarino52
Estimated H-index: 52
(University of Perugia),
Claudia Volpi21
Estimated H-index: 21
(University of Perugia)
+ 11 AuthorsPaolo Puccetti71
Estimated H-index: 71
(University of Perugia)
Excessive glutamate seen in multiple sclerosis leads to excitotoxicity and neuronal dysfunction. Fallarino et al. find that the clinical signs and neuroinflammation in experimental autoimmune encephalitis is worsened in mice deficient in the metabotropic glutamate receptor-4. Small molecules that enhance signaling through this receptor suppress neuroinflammation by promoting T regulatory cell development and suppressing TH17 responses. This cross-talk between the nervous and immune system sugges...
Published on Apr 1, 2008in Toxicologic Pathology1.38
Santokh Gill15
Estimated H-index: 15
,
Michael Barker1
Estimated H-index: 1
,
Olga Pulido20
Estimated H-index: 20
Glutamate receptors (GluRs) have been implicated in brain function and pathology. Their presence in peripheral tissues suggests a vital role in the pathophysiology of various organ systems. In earlier studies, the authors reported the differential distribution of ionotropic and metabotropic GluRs in neural and nonneural peripheral tissues of the rat. In this study, they investigated the presence and the localization of the GluRs in the reproductive organs of Macaca fascicularis. The data illustr...
Published on Feb 1, 2008in Pakistan Journal of Nutrition
A.O. Nwaopara2
Estimated H-index: 2
(Ambrose Alli University),
M.A.C. Odike2
Estimated H-index: 2
(Ambrose Alli University)
+ 2 AuthorsG.I. Ewere1
Estimated H-index: 1
(Ambrose Alli University)
Allergic reactions have been associated with cloves, black pepper and mast cells. The presence of mast cells and renal fibrosis has also been reported and in line with an ongoing investigation on the effects of excessive consumption of Yaji the complex Nigerian meat sauce, this study was designed to correlate the individual and combined effects of the excessive consumption of Yaji-spices (ginger, clove, red pepper and black pepper) on the histology of the kidney, using 12 adult rabbits. The anim...
Published on Dec 1, 2007in Neuropharmacology4.37
Sam M. Greenwood9
Estimated H-index: 9
(Strathclyde Institute of Pharmacy and Biomedical Sciences),
Christopher N. Connolly31
Estimated H-index: 31
(Dund.: University of Dundee)
Glutamate is the predominant excitatory neurotransmitter in the mammalian central nervous system (CNS) and is normally stored intracellularly. However, in instances of CNS injury or disease, increased concentrations of extracellular glutamate can result in the over-activation of ionotropic glutamate receptors and trigger neuronal cell death (termed excitotoxicity). Two early hallmarks of such neuronal toxicity are mitochondrial dysfunction (depolarisation, decreased ATP synthesis, structural col...
Published on Jun 1, 2007in Pakistan Journal of Nutrition
A.O. Nwaopara2
Estimated H-index: 2
,
M.A.C. Odike2
Estimated H-index: 2
+ 1 AuthorsM.I. Adoye1
Estimated H-index: 1
Yaji, otherwise ‘suya sauce’, used in serving the meat delicacy called ‘Suya’, is a complex mixture of spices and additives whose active ingredients on individual basis, are known to have side effects if consumed in excess. The growing concern is that the excessive consumption of Yaji signifies an excessive consumption of a combination of these constituents amongst which are ginger, cloves, red pepper and black pepper; which are mixed without a standardized format. This study was therefore desig...
Published on Aug 8, 2007in Molecular and Cellular Biochemistry2.88
Voja Pavlovic7
Estimated H-index: 7
(University of Niš),
Dusica Pavlovic15
Estimated H-index: 15
(University of Niš)
+ 4 AuthorsDragana Velickovic1
Estimated H-index: 1
(University of Niš)
It has been demonstrated that administration of high concentrations of monosodium glutamate (MSG), induce oxidative stress in different organs, but not in thymus. In the present study we examined the role of oxidative stress in MSG-induced thymocyte apoptosis. MSG was administrated intraperitoneally (4 mg/g of body weight) for six consecutive days. Animals were sacrificed at 1st, 7th, and 15th day after last MSG dose. MSG administration to animals significantly increased apoptotic rate of thymoc...
Published on Feb 1, 2007in Current Opinion in Pharmacology5.20
Urs F. Greber58
Estimated H-index: 58
(UZH: University of Zurich),
Christine E. Gee13
Estimated H-index: 13
(UZH: University of Zurich),
Pascal Benquet18
Estimated H-index: 18
(University of Rennes)
Metabotropic glutamate receptors are classified into three groups, primarily on the basis of sequence similarity and whether they positively couple to the phospholipase C cascade or negatively couple to adenylyl cyclases. The past decade of research, drawing on sophisticated molecular approaches, has revealed a multitude of additional intracellular components that assemble as protein scaffolds around neuronal metabotropic glutamate receptors, establishing functional links to postsynaptic density...
Published on Jan 1, 2007in Acta Neurobiologiae Experimentalis1.53
Monika Liguz-Lecznar8
Estimated H-index: 8
,
Jolanta Skangiel-Kramska17
Estimated H-index: 17
Glutamate is the predominant excitatory neurotransmitter in the central nervous system (CNS) and glutamatergic transmission is critical for controlling neuronal activity. Glutamate is stored in synaptic vesicles and released upon stimulation. The homeostasis of glutamatergic system is maintained by a set of transporters present in plasma membrane and in the membrane of synaptic vesicles. The family of vesicular glutamate transporters in mammals is comprised of three highly homologous proteins: V...
Published on Dec 31, 2006in The Internet Journal of Health
Andrew O. Eweka2
Estimated H-index: 2
The effect of monosodium glutamate, used as food additive on the Kidneys of adult wistar rat was investigated. Both adult male and female Wistar rats (n=24) average weight of 185g were randomly assigned into two treatments (n=12) and control (n=6) groups. The rats in the treatment groups received 3g and 6g of Monosodium glutamate thoroughly mixed with the grower's mash daily for fourteen days. The control rats received equal amount of the grower's mash without Monosodium glutamate added daily. T...
Published on Aug 1, 2006in Toxicologic Pathology1.38
Susan A. Elmore20
Estimated H-index: 20
The spleen is the largest secondary lymphoid organ, is considered the draining site for compounds that are administered intravenously, and is therefore considered an important organ to evaluate for treatment-related lesions. Due to the presence of B and T lymphocytes, the immunotoxic effects of xenobiotics or their metabolites on these cell populations may be reflected in the spleen. Therefore it is one of the recommended organs to evaluate for enhanced histopathology of the immune system. The t...
Cited By4
Newest
Published on May 1, 2016in Food and Chemical Toxicology3.77
Nazmiye Ataseven1
Estimated H-index: 1
(Gazi University),
Deniz Yüzbaşıoğlu14
Estimated H-index: 14
(Gazi University)
+ 1 AuthorsFatma Ünal14
Estimated H-index: 14
(Gazi University)
Monosodium glutamate (MSG) is one of the most widely used flavor enhancers throughout the world. The aim of this study is to investigate the genotoxic potential of MSG by using chromosome aberrations (CAs), sister-chromatid exchanges (SCEs), cytokinesis-blocked micronucleus (CBMN), and random amplified polymorphic DNA-polimerase chain reaction (RAPD-PCR) in cultured human lymphocytes and alkaline comet assays in isolated human lymphocytes, which were incubated with six concentrations (250, 500, ...
Published on Jan 1, 2015
Vandana Kumbhare1
Estimated H-index: 1
,
Ujwal Gajbe1
Estimated H-index: 1
+ 2 AuthorsSamarth Shukla
Introduction: Monosodium Glutamate, also known as MSG. [1-3] Monosodium glutamate is a controversial additive. It is metabolized in liver and kidney plays an important role in its elimination. [4] In 1968 Schaumburg H.H and R. Byck [5] were the first people to draw attention towards Chinese restaurant syndrome characterized by headache, chest discomfort and facial flushing. [6, 7, 8] The diversity in manifestation of toxic effects and susceptibility of different species of animals to MSG was suc...
Published on Jan 1, 2014in Journal of Cytology and Histology
Zeinab A. Hassan2
Estimated H-index: 2
,
Manar Hamed Arafa5
Estimated H-index: 5
+ 2 AuthorsHanan Fathy Al-Saeed1
Estimated H-index: 1
Monosodium glutamate (MSG), a flavor enhancer, is used in modern nutrition to improve food palatability. The objectives of the current study were to investigate the effect of MSG on thymus as well as spleen structures and functions. Also, to evaluate the possibility of recovery after cessation of administration. Adult male rats were divided into three groups: control, MSG (3 g MSG/kg body weight daily for 8 weeks by oral gavages), and Recovery (MSG for same period and then left untreated for add...
View next paperOrgan toxicity of monosodium glutamate in adult albino Wistar rats