Original paper
Differences in secondary structure between packaged and unpackaged single-stranded DNA of bacteriophage .vphi.X174 determined by Raman spectroscopy: a model for .vphi.X174 DNA packaging
Abstract
The single-stranded packaged genome (ssDNA) of bacteriophage phi X174 is shown by Raman spectroscopy to lack both the ordered phosphodiester backbone and base stacking, which are demonstrated for unpackaged, protein-free ssDNA. In solutions of moderate ionic strength, unpackaged ssDNA contains 36 +/- 7% of deoxyribosyl phosphate groups with conventional B-type backbone geometry [i.e., gauche- and trans orientations, respectively, for the 5'O-P...
Paper Details
Title
Differences in secondary structure between packaged and unpackaged single-stranded DNA of bacteriophage .vphi.X174 determined by Raman spectroscopy: a model for .vphi.X174 DNA packaging
Published Date
May 1, 1991
Journal
Volume
30
Issue
20
Pages
4855 - 4863
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Notes
History