Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
Abstract
We describe a modification of two-dimensional (2-D) polyacrylamide gel electrophoresis that requires only a single gel to reproducibly detect differences between two protein samples. This was accomplished by fluorescently tagging the two samples with two different dyes, running them on the same 2-D gel, post-run fluorescence imaging of the gel into two images, and superimposing the images. The amine reactive dyes were designed to insure that...
Paper Details
Title
Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
Published Date
Jan 1, 1997
Journal
Volume
18
Issue
11
Pages
2071 - 2077
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