Cell death and cell proliferation in cartilage layers in human anterior cruciate ligament tibial insertions after rupture

Published on Aug 1, 2010in Connective Tissue Research2.167
· DOI :10.3109/03008200903318303
Hirotaka Mutsuzaki12
Estimated H-index: 12
(Ibaraki Prefectural University of Health Sciences),
Masataka Sakane19
Estimated H-index: 19
(University of Tsukuba)
+ 3 AuthorsNaoyuki Ochiai32
Estimated H-index: 32
(University of Tsukuba)
The purpose of this study is to investigate cellular responses and histological changes of cartilaginous layers in human anterior cruciate ligament (ACL) tibial insertions after rupture compared with those in normal insertions. Fully 16 tibial insertions of ruptured ACLs were obtained during primary ACL reconstructions. We also obtained 16 normal ACL tibial insertions from cadavers. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) to detect apoptosis, proliferating cell nuclear antigen (PCNA) staining, and histological examination were performed. The percentage of TUNEL-positive chondrocytes in ruptured ACL insertions (30.2 ± 15.6%) was higher than that in normal insertions (9.6 ± 5.8%). The percentage of PCNA-positive chondrocytes was significantly different between ruptured ACL insertions (19.9 ± 15.0%) and normal insertions (12.3 ± 7.3%). The average thickness of the cartilage layer, the glycosaminoglycan-stained area, and the number of chondrocy...
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