PYRIDOXINE REQUIREMENT OF JUVENILE ABALONE, HALIOTIS DISCUS HANNAI INO
Published on Sep 1, 2007in Journal of Shellfish Research1.04
· DOI :10.2983/0730-8000(2007)26[815:PROJAH]2.0.CO;2
Juvenile abalone, Haliotis discus hannai Ino (initial mean body weight: 0.56 ± 0.01 g, shell length: 15.74± 0.13 mm) were fed six diets with graded levels of pyridoxine (PN) (0.40, 12.5, 22.7, 43.5, 87.8, and 172.2 mg/kg diet). Each diet was fed to three replicate groups of juvenile abalone for 16 wk. Dietary PN significantly (P < 0.05) affected abalone growth, tissue concentrations of PN and pyridoxal 5#-phosphate (PLP), and the activities of asparatate aminotransferase (AST) and alanine aminotransferase (ALT). The responses of all these parameters to dietary pyridoxine levels fitted the broken-line model. Broken- line regression analyses showed that the breakpoints were 23 on the basis of growth data, 32-38 on tissue concentrations of PN and PLP, 20-21 on the activities of visceral AST and ALT, and 39-40 on the activities of muscle AST and ALT. Hence, the requirement of H. discus hannai for dietary pyridoxine should be 23 mg/kg diet for maximum growth, and about 40 mg/kg diet for the saturation of tissue PN and PLP, or tissue aminotransferase activities.