Propofol Activates and Allosterically Modulates Recombinant Protein Kinase Cϵ

Background Myocardial protection by anesthetics is known to involve activation of protein kinase C epsilon (PKC epsilon). A key step in the activation process is autophosphorylation of the enzyme at serine 729. This study's objectives were to identify the extent to which propofol interacts with PKC epsilon and to identify the molecular mechanism(s) of interaction. Methods Immunoblot analysis of recombinant PKC epsilon was used to assess...