Simultaneous determination of catecholamines and their metabolites related to Alzheimer's disease in human urine

A simple and specific high‐performance liquid chromatography method coupled with fluorescence detection (HPLC‐FL) has been developed for the simultaneous determination of L ‐3,4‐dihydroxyphenylalanine, norepinephrine, dopamine, epinephrine and 3,4‐dihydroxyphenylacetic acid in human urine. The samples were derivatized by 1,2‐diphenylethylenediamine with isoprenaline as internal standard. The factors affecting the fluorescence yield were...