Match!

QX-like infectious bronchitis virus isolated from cases of proventriculitis in commercial broilers in England

Published on Dec 8, 2012in Veterinary Record2.101
· DOI :10.1136/vr.101005
Kannan Ganapathy13
Estimated H-index: 13
(University of Liverpool),
M. Wilkins1
Estimated H-index: 1
(University of Liverpool)
+ 4 AuthorsRichard C. Jones35
Estimated H-index: 35
(University of Liverpool)
Abstract
This short communication reports on the isolation of QX-like infectious bronchitis virus (IBV) from the proventriculus of broiler chicken in England, and its pathogenesis in specific-pathogen-free (SPF) chicks. This appears to be the first report of the isolation of this virus from the proventriculus of affected chicks in the UK. The virus was first recovered from this tissue in chickens in China in 1996 (YuDong and others 1998), and later, more cases were reported, including in flocks vaccinated against IBV (Yu and others 2001). We received samples of kidney and proventriculus from a flock of 56-days-old commercial broiler chickens, where the submitting field veterinarian reported respiratory signs, increased levels of mortality, a poor feed conversion ratio and suboptimal live body weight gain. At necropsy, proventriculitis and swollen kidneys were reported. Similar tissues were pooled and processed for IBV detection by reverse-transcriptase PCRs (RT-PCR), as previously described (Worthington and others 2008), and virus isolation (VI) were attempted. RT-PCR tests on the supernatant of the pooled tissues of kidneys were negative for IBV, while the proventriculus samples were positive. The proventriculus was negative for avian metapneumovirus (aMPV), Newcastle disease virus (NDV) and …
  • References (7)
  • Citations (14)
📖 Papers frequently viewed together
144 Citations
170 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References7
Newest
#1J. J. de WitH-Index: 16
Last. T. H. F. FabriH-Index: 5
view all 6 authors...
Since the end of 2003, strains of the D388 serotype (QX genotype) of infectious bronchitis virus (IBV) have caused considerable damage to the Dutch poultry industry. In order to better understand the pathogenesis of infection caused by this infectious bronchitis variant and to be able to support the poultry industry with substantiated advice to prevent or decrease the damage caused by the D388 strain, several vaccination and challenge experiments were performed in young specific pathogen free la...
39 CitationsSource
#1Zsófia Benyeda (Szent István University)H-Index: 4
#2Tamás Mató (CEVA Logistics)H-Index: 15
Last. Vilmos Palya (CEVA Logistics)H-Index: 21
view all 8 authors...
69 CitationsSource
#1Calogero TerreginoH-Index: 28
#2Anna ToffanH-Index: 17
Last. Ilaria CapuaH-Index: 49
view all 9 authors...
The aims of this study were firstly to evaluate the pathogenicity of an Italian isolate of the QX strain of infectious bronchitis (IB) virus using 1-day-old female specific pathogen free chicks (layer type) and 1-day-old female commercial broiler type chickens, and secondly to assess the level of protection induced in these birds by a vaccination programme including the IB Massachusetts and 4/91 serotype live attenuated vaccines. Unvaccinated birds showed clinical signs of varying severity, pred...
89 CitationsSource
#1K. J. Worthington (University of Liverpool)H-Index: 10
#2R. J. W. CurrieH-Index: 1
Last. Richard C. Jones (University of Liverpool)H-Index: 35
view all 3 authors...
144 CitationsSource
#1R. E. GoughH-Index: 14
#2W. J. CoxH-Index: 2
Last. Richard C. Jones (University of Liverpool)H-Index: 35
view all 5 authors...
SIR, — We wish to report the isolation and identification of a strain of infectious bronchitis virus (ibv) which shows a close genetic relationship with the so-called Chinese qx strain of ibv. As far as we are aware, this is the first report of the presence of this virus in the uk. A six- to
42 CitationsSource
#1Li YuH-Index: 51
#2Yuming JiangH-Index: 26
Last. Kwangac JH-Index: 1
view all 7 authors...
Outbreaks of an avian disease in infectious bronchitis-vaccinated chickens in China have led to the characterization of coronaviral isolates Q1, J2, and T3, which were isolated from proventricular tissues of the affected young layer flocks. Serologic analysis revealed that they could induce high titers of infectious bronchitis virus (IBV) antibodies in inoculated specificpathogen-free (SPF) chickens in indirect enzyme-linked immunosorbent assay but were not neutralized by antisera specific to th...
108 CitationsSource
#1Wang YudongH-Index: 2
#2Wang YongLinH-Index: 1
Last. Wang ShuShuangH-Index: 1
view all 8 authors...
61 Citations
Cited By14
Newest
#1Andrea Laconi (UU: Utrecht University)H-Index: 1
#2E.A.W.S. Weerts (UU: Utrecht University)
Last. Monique H. Verheije (UU: Utrecht University)H-Index: 22
view all 8 authors...
Abstract Infectious bronchitis (IB) is a highly contagious respiratory disease of poultry, caused by the avian coronavirus infectious bronchitis virus (IBV). Currently, one of the most relevant genotypes circulating worldwide is IBV-QX (GI-19), for which vaccines have been developed by passaging virulent QX strains in embryonated chicken eggs. Here we explored the attenuated phenotype of a commercially available QX live vaccine, IB Primo QX, in specific pathogens free broilers. At hatch, birds w...
Source
#1Michael Haryadi Wibowo (UGM: Gadjah Mada University)H-Index: 3
#2Teridah Ernala Ginting (University of Pelita Harapan)H-Index: 1
Last. Widya Asmara (UGM: Gadjah Mada University)H-Index: 5
view all 3 authors...
Source
#1Christopher Ball (University of Liverpool)H-Index: 3
#2Anne Forrester (University of Liverpool)H-Index: 6
Last. Kannan Ganapathy (University of Liverpool)H-Index: 13
view all 3 authors...
Abstract Respiratory diseases continue to have a major impact on poultry health, welfare and productivity. However, little information is available on their current status in UK poultry flocks. We investigated the presence of four economically important respiratory pathogens in healthy or problematic flocks; infectious bronchitis virus (IBV), avian metapneumovirus (aMPV), Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae (Ms). Samples from 131 UK poultry flocks were received during the 12 mo...
1 CitationsSource
#1Rajesh Chhabra (University of Liverpool)H-Index: 6
#2Christopher Ball (University of Liverpool)H-Index: 3
Last. Kannan Ganapathy (University of Liverpool)H-Index: 13
view all 4 authors...
Abstract Avian infectious bronchitis virus (IBV) continues to cause serious economic losses in global chicken production. Concurrent circulation of both classic and variant IBVs have been identified in most parts of the world, raising major challenges to global prevention and control efforts. Therefore, immunopathogenesis, particularly early host responses, needs to be better understood for effective control of diseases caused by different strains of IBVs. We investigated differing immunopathoge...
6 CitationsSource
#1Basim Manswr (University of Liverpool)H-Index: 1
#2Christopher Ball (University of Liverpool)H-Index: 3
Last. Kannan Ganapathy (University of Liverpool)H-Index: 13
view all 5 authors...
ABSTRACTSequence variability in the S1 gene determines the genotype of infectious bronchitis virus (IBV) strains. A single RT-PCR assay was developed to amplify and sequence the full S1 gene for six classical and variant IBVs (M41, D274, 793B, IS/885/00, IS/1494/06 and Q1) enriched in allantoic fluid (AF) or the same AF inoculated onto Flinders Technology Association (FTA) cards. Representative strains from each genotype were grown in specific-pathogen-free eggs and RNA was extracted from AF. Fu...
3 CitationsSource
#1Claudia Carranza (USP: University of São Paulo)H-Index: 3
#2Claudete S. Astolfi-Ferreira (USP: University of São Paulo)H-Index: 8
Last. Antonio José Piantino Ferreira (USP: University of São Paulo)H-Index: 24
view all 9 authors...
1. Infectious bronchitis virus (IBV) variants in Brazil were isolated during 2010-2015 for epidemiological and molecular analysis to characterise the different variants and perform a bioinformatic analysis to compare with sequences of variants collected over the previous 40 years. 2. Of the 453 samples examined, 61.4% were positive for IBV and 75.9% of these were considered to have the BR-I genotype and were detected in birds of all ages distributed in all five Brazilian regions. 3. The ratio of...
3 CitationsSource
Infectious bronchitis virus (IBV) variants constantly emerge and pose economic threats to poultry farms worldwide. Numerous studies on the molecular and pathogenic characterization of IBV variants have been performed between 2007 and 2017, which we have reviewed herein. We noted that viral genetic mutations and recombination events commonly gave rise to distinct IBV genotypes, serotypes and pathotypes. In addition to characterizing the S1 genes, full viral genomic sequencing, comprehensive antig...
12 CitationsSource
#1Faruku Bande (UPM: Universiti Putra Malaysia)H-Index: 6
#2Siti Suri Arshad (UPM: Universiti Putra Malaysia)H-Index: 12
Last. Venugopal Nair (Institute for Animal Health)H-Index: 32
view all 6 authors...
The poultry industry faces challenge amidst global food security crisis. Infectious bronchitis is one of the most important viral infections that cause huge economic loss to the poultry industry worldwide. The causative agent, infectious bronchitis virus (IBV) is an RNA virus with great ability for mutation and recombination; thus, capable of generating new virus strains that are difficult to control. There are many IBV strains found worldwide, including the Massachusetts, 4/91, D274, and QX-lik...
12 CitationsSource
#1A. I. AdebiyiH-Index: 1
#2A. F. FagbohunH-Index: 1
1 CitationsSource
#1Vishwanatha Reddy Avalakuppa Papi Reddy (UGent: Ghent University)H-Index: 3
#2Ivan Trus (UGent: Ghent University)H-Index: 4
Last. Hans Nauwynck (UGent: Ghent University)H-Index: 57
view all 6 authors...
In the present study, the replication kinetics of nephropathogenic (B1648) and respiratory (Massachusetts-M41) IBV strains were compared in vitro in respiratory mucosa explants and blood monocytes (KUL01+ cells), and in vivo in chickens to understand why some IBV strains have a kidney tropism. B1648 was replicating somewhat better than M41 in the epithelium of the respiratory mucosa explants and used more KUL01+ cells to penetrate the deeper layers of the respiratory tract. B1648 was productivel...
6 CitationsSource