The Implications of Proteolytic Background for Shotgun Proteomics
Abstract
The analysis by liquid chromatography coupled to tandem mass spectrometry of complex peptide mixtures, generated by proteolysis of protein samples, is the main proteomics method used today. The approach is based on the assumption that each protein present in a sample reproducibly and predictably generates a relatively small number of peptides that can be identified by mass spectrometry. In this study this assumption was examined by a targeted...
Paper Details
Title
The Implications of Proteolytic Background for Shotgun Proteomics
Published Date
Sep 1, 2007
Volume
6
Issue
9
Pages
1589 - 1598
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