Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Abstract
Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically dead Cas9 lacking endonuclease activity, when coexpressed with a guide RNA, generates a DNA recognition complex that can specifically interfere with...
Paper Details
Title
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Published Date
Feb 1, 2013
Journal
Volume
152
Issue
5
Pages
1173 - 1183
Citation AnalysisPro
You’ll need to upgrade your plan to Pro
Looking to understand the true influence of a researcher’s work across journals & affiliations?
- Scinapse’s Top 10 Citation Journals & Affiliations graph reveals the quality and authenticity of citations received by a paper.
- Discover whether citations have been inflated due to self-citations, or if citations include institutional bias.
Notes
History