Development of a Real-time PCR test for porcine group A rotavirus diagnosis
Abstract
Group A Rotavirus (RVA) is one of the most common causes of diarrhea in humans and several animal species. A SYBR-Green Real-Time polymerase chain reaction (PCR) was developed to diagnose RVA from porcine fecal samples, targeting amplification of a 137-bp fragment of nonstructural protein 5 (NSP5) gene using mRNA of bovine NADH-desidrogenase-5 as exogenous internal control. Sixty-five samples were tested (25 tested positive for conventional PCR...
Paper Details
Title
Development of a Real-time PCR test for porcine group A rotavirus diagnosis
Published Date
Jan 1, 2015
Volume
35
Issue
1
Pages
39 - 43
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