RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
Abstract
We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1...
Paper Details
Title
RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
Published Date
Jul 1, 2014
Journal
Volume
4
Issue
7
Pages
1291 - 1295
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Notes
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