Enhancer Control of Local Accessibility to V(D)J Recombinase

We have studied the role of transcriptional enhancers in providing recombination signal sequence (RSS) accessibility to V(D)J recombinase by examining mice carrying a transgenic human T-cell receptor (TCR) δ gene minilocus. This transgene is composed of unrearranged variable (Vδ1 and Vδ2), diversity (Dδ3), joining (Jδ1 and Jδ3), and constant (Cδ) gene segments. Previous data indicated that with the TCR δ enhancer (Eδ) present in the Jδ3–Cδ...