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Antiproliferative and cytotoxic effect of a novel organotin compound on mammalian cells both in vitro and in vivo

Published on Jan 1, 2002in Mutation Research-genetic Toxicology and Environmental Mutagenesis2.256
· DOI :10.1016/S1383-5718(01)00289-3
Christine Syng-ai3
Estimated H-index: 3
(NEHU: North Eastern Hill University),
Tushar S. Basu Baul23
Estimated H-index: 23
(NEHU: North Eastern Hill University),
Anupam Chatterjee17
Estimated H-index: 17
(NEHU: North Eastern Hill University)
Abstract
Abstract Organotin compounds are organometallic compounds showing various toxicological properties. Several organotin compounds also showed an antineoplastic effect. However, their relative mutagenic potential is not well established. In this study Et 2 SnCl 2 ·L [L= N -(2-pyridylmethylene)-4-toluidine] (OTC) has been subjected to investigation for its cytotoxic effect in mouse bone marrow cells (BMCs) and human peripheral blood lymphocyte cells (HPBLs). The SnN bond in OTC is 2.46 A which is greater than 2.39 A and therefore, a better formation of tin–DNA complex can be expected. The present data indicate that OTC induced significant delay in cell kinetics and sister chromatid exchanges (SCEs) in both BMCs and HPBLs, whereas, induction of chromosome aberrations was found only in HPBLs. The presence of buthionine sulfoximine (BSO) modulated cellular sensitivity towards OTC in both cell systems. It may be inferred that the OTC could bind on DNA more easily owing to its structural advantage and this may explain the induction of DNA damage and the delay in cell proliferation. Since the cytotoxic effect of OTC is more in glutathione depleted cells, the concentration of OTC may be reduced to get an antitumour effect in GSH-depleted cells and thus minimizes its toxic side effect.
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Abstract Two organotin pesticides, triphenyltin acetate (TPTA) and triphenyltin hydroxide (TPTH), were evaluated for their ability to induce micronuclei (MN) and sister chromatid exchange (SCE) in vitro using cultured Chinese hamster ovary (CHO) cells and in vivo BALB/c mouse erythrocytes. Both pesticides induced a dose-dependent increase but only TPTH induced a significant increase in MN at the highest dose (150 ng/ml) tested in CHO cells. With adding S9 microsomal fractions, both pesticides in...
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enzyme c-glutamylcysteine synthetase and increases Purpose: Chromosome aberrations (CA) were used as an end- cellular radiosensitivity. GSH is an important factor point to investigate the eU ect of buthionine sulphoximine (BSO), in the radiosensitization of hypoxic cells and it has a potent glutathione-depleting agent, on the radiosensitivity of been described as a chemical repair agent in mammamammalian cells. The aim was to obtain information about the lian cells (Midander 1982, Clark et al. 1...
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