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PFP1, a gene encoding an Epc-N domain-containing protein, is essential for pathogenicity of the barley pathogen Rhynchosporium commune.

Published on Aug 1, 2014in Eukaryotic Cell
· DOI :10.1128/EC.00043-14
Sylvia Siersleben1
Estimated H-index: 1
(Leibniz Association),
Daniel Penselin4
Estimated H-index: 4
(Leibniz Association)
+ 2 AuthorsWolfgang Knogge18
Estimated H-index: 18
(Leibniz Association)
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Abstract
Scald caused by Rhynchosporium commune is an important foliar disease of barley. Insertion mutagenesis of R. commune generated a nonpathogenic fungal mutant which carries the inserted plasmid in the upstream region of a gene named PFP1. The characteristic feature of the gene product is an Epc-N domain. This motif is also found in homologous proteins shown to be components of histone acetyltransferase (HAT) complexes of fungi and animals. Therefore, PFP1 is suggested to be the subunit of a HAT complex in R. commune with an essential role in the epigenetic control of fungal pathogenicity. Targeted PFP1 disruption also yielded nonpathogenic mutants which showed wild-type-like growth ex planta, except for the occurrence of hyphal swellings. Complementation of the deletion mutants with the wild-type gene reestablished pathogenicity and suppressed the hyphal swellings. However, despite wild-type-level PFP1 expression, the complementation mutants did not reach wild-type-level virulence. This indicates that the function of the protein complex and, thus, fungal virulence are influenced by a position-affected long-range control of PFP1 expression.
  • References (63)
  • Citations (4)
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References63
Newest
#1Hong Wu (SGC: Structural Genomics Consortium)H-Index: 19
#2Hong Zeng (SGC: Structural Genomics Consortium)H-Index: 14
Last.Jinrong Min (U of T: University of Toronto)H-Index: 36
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#1Ralf Horbach (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 7
#2Aura Rocio Navarro-Quesada (Leibniz Association)H-Index: 1
Last.H. B. Deising (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 32
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Cited By4
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#1Juan S. Ramirez-Prado (KAUST: King Abdullah University of Science and Technology)H-Index: 5
#2Sophie J. M. Piquerez (INRA: Institut national de la recherche agronomique)H-Index: 1
Last.Moussa Benhamed (INRA: Institut national de la recherche agronomique)H-Index: 21
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#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
#2Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
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