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Comparison of immunological properties of various bioactive combinations.

Published on Sep 1, 2012
· DOI :10.5507/bp.2012.065
Vaclav Vetvicka28
Estimated H-index: 28
,
Jana Vetvickova16
Estimated H-index: 16
Abstract
Background: Lately, more and more preparation of various cocktails or mixtures of bioactive modulators have been introduced. Their true activity is, however, rarely tested. Aim: To compare six commercially available, glucan-based immunostimulators. Methods: Immunological effects of tested combinations were measured by evaluation of phagocytosis of synthetic particles by peripheral blood neutrophils, production of IL-2 by mouse splenocytes, production of superoxide anion and nitrite oxide, antibody response to imunization with ovalbumin, and NK cell activity. Results: Our results showed that with the exception of the highest doses (phagocytosis) and superoxide anion and nitrite oxide production, only RVB 300 showed significant immunostimulative activity. Conclusion: Based on our results, we can conclude that most of the tested natural immunomodulators have limited, if any, biological effects. Only RVB 300 significantly stimulated all six tested immunological reactions.
  • References (14)
  • Citations (5)
References14
Newest
#1Mamdooh Ghoneum (Charles R. Drew University of Medicine and Science)H-Index: 23
#2Sudhanshu Agrawal (UCI: University of California, Irvine)H-Index: 22
Arabinoxylan rice bran (MGN-3/Biobran) is a potent biological response modifier (BRM) that activates natural killer (NK) cells, T cells and monocytes. Currently, little is known regarding the effec...
28 CitationsSource
#1Mamdooh Ghoneum (UCLA: University of California, Los Angeles)H-Index: 23
#2Sastry GollapudiH-Index: 31
owever, a synergistic effect was noticed post-treatment with both agents that maximized at 100μg/m l MGN-3 plus 10μM curcumin. his synergy was characterized by an 87% decrease in cell number and a 2.6 fold increase in t he percentage of apoptotic U266 cells. Cell cycle analysis showed a 53% decrease in the percentage of cells in th e G0-G1 phase treated with MGN-3 and curcumin (from 36% to 17%). Analysis of the expression of the pro and antiapoptotic m olecules Bax and Bcl-2 revealed synergistic...
21 CitationsSource
#1Vaclav VetvickaH-Index: 28
#2Roberto BaigorriH-Index: 18
Last. Jean-Claude YvinH-Index: 20
view all 5 authors...
Abstract Humic acids are compounds resulting from decomposition of organic matter. Despite their common presence, our knowledge of their biological effects is limited, and current findings are controversial. We decided to evaluate the immunological effects of two different types of humic acids, differing in source and biochemical characteristics. Using both components either alone or in combination with the well-established yeast-derived immunomodulator glucan, we measured their effects on both ...
18 CitationsSource
#1Haibin Huang (UMMS: University of Massachusetts Medical School)H-Index: 19
#2Gary R. Ostroff (UMMS: University of Massachusetts Medical School)H-Index: 27
Last. Stuart M. Levitz (UMMS: University of Massachusetts Medical School)H-Index: 64
view all 5 authors...
Glucan particles (GPs) are purified Saccharomyces cerevisiae cell walls treated so that they are primarily 1,3-D- glucans and free of mannans and proteins. GPs are phagocytosed by dendritic cells (DCs) via the Dectin-1 receptor, and this in- teraction stimulates proinflammatory cytokine secretion by DCs. As the hollow, porous GP structure allows for high antigen loading, we hypothesized that antigen-loaded GPs could be exploited as a receptor-targeted vaccine delivery system. Ovalbumin (OVA) was...
93 CitationsSource
#1Jessica R. Nerren (ARS: Agricultural Research Service)H-Index: 12
#2Michael H. Kogut (ARS: Agricultural Research Service)H-Index: 41
Abstract A critical component of host innate immunity is recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs). Dectin-1 is the primary PRR for exogenous β-glucan, a component of fungal and bacterial cell walls. A previous study conducted in our laboratory demonstrated that administration of β-glucan as a feed additive resulted in increased innate immune function of neonatal chickens, suggesting that chickens possess a Dectin-1-like β-glucan recept...
27 CitationsSource
#1Vaclav Vetvicka (University of Louisville)H-Index: 28
#2Bohuslav Dvorak (UA: University of Arizona)H-Index: 30
Last. Jean-Claude YvinH-Index: 20
view all 7 authors...
d-Glucans represent highly conserved structural components of cell walls in yeast, fungi, or seaweed. However, it is still unknown how they mediate their effects. The aim of this study was to evaluate both intraperitoneal and oral application of seaweed-derived (1 → 3)--d-glucan Phycarine. Phycarine showed significant stimulation of phagocytosis by peripheral blood cells. In addition, the efficiency of chemotherapy of Lewis lung carcinoma with cyclophosphamide was potentiated by Phycarine admini...
85 CitationsSource
Abstract Glucans have a long history as nonspecific biological modulators. A novel glucan—Phycarine—was isolated from sporophytes of Laminaria digitata . Phycarine showed significant stimulation of phagocytic activity as well as potentiation of synthesis and release of IL-1, IL-6 and TNF-α. In addition, Phycarine increased NK cell-mediated killing of tumor cells both in vitro and in vivo while acting via complement receptor type 3 (CR3) receptors.
119 CitationsSource
#1G. Ditteová (SAV: Slovak Academy of Sciences)H-Index: 1
#2S. VelebnýH-Index: 1
Last. G. HrčkovaH-Index: 1
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The effects of glucan and liposomized glucan, alone or co-administered with vitamin C, and empty liposomes on hepatic fibrosis in mice infected with Mesocestoides corti (M. vogae ) tetrathyridia were studied. Preparations were administered every third day from day 7 to day 31 post-infection (p.i.), nine doses in total. Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and cholesterol levels were measured in sera collected on days 11, 15, 21, 28, 32, 42, 50 and 65 p.i...
15 CitationsSource
#1R.M. BrinkebornH-Index: 1
#2D.V. ShahH-Index: 1
Last. F.H. DegenringH-Index: 1
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Summary The aim of this randomized, double-blind, placebo controlled study was to investigate the efficacy and safety of different doses and preparations of Echinacea purpurea in the treatment of common cold. 246 of 559 recruited healthy, adult volunteers caught a common cold and took 3 times daily 2 tablets of either Echinaforce® ( Echinacea purpurea -preparation from 95% herba and 5% radix), Echinacea purpurea concentrate (same preparation at 7 times higher concentration), special Echinacea pu...
104 CitationsSource
#1Viviane Verlhac (Hoffmann-La Roche)H-Index: 9
#2Alex ObachH-Index: 20
Last. Reid HoleH-Index: 2
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Abstract The aim of this study was to determine the influence of dietary glucan and vitamin C, alone and in combination, on the cellular and humoral immune responses of rainbow trout. After 4 weeks of adaptation on a control diet containing glucan at 0 ppm and vitamin C at 150 ppm, four groups of rainbow trout were fed the experimental diets containing vitamin C at 150 and 1000 ppm plus a fixed amount of glucan, or no glucan for 2 weeks. The fish were then switched back to the control diet. Non-...
131 CitationsSource
Cited By5
Newest
#1Vaclav Vetvicka (University of Louisville)H-Index: 28
#2Jana Vetvickova (University of Louisville)H-Index: 16
Natural immunomodulators are getting more and more popular. The popularity, however, often brings over- optimistic claims and mediocre effects. The purpose of the present study was to directly compare eleven most commonly used immunomodulators. Through testing both cellular and humoral branches of immune reactions, we found that most of the immunomodulators tested have limited, if any, effects, with glucan being consistently the most active molecule strongly stimulating every reaction evaluated....
6 Citations
#1Story JennyH-Index: 1
#2Vetvicka VaclavH-Index: 3
Last. Angove MichaelH-Index: 1
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β1,3-glucans from fungi, cereals, seaweeds and bacte ria have been shown to possess favourable biologica l and anti-carcinogenic activities including upregula tion of phagocytosis, cytokine production enhanceme nt, superoxide and nitrite production; antibody secreti on and stimulation of signalling pathways associate d with proto-oncogene expression. However, human dietary supplements containing β1,3-glucans vary in efficacy due to glucan source, the lifecycle stage of the so urce at extraction,...
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The search for an optimal combination of natural im munomodulators led us to study the biological effec ts of the combination of a cinnamon extract Cinnulin PF a nd probiotic LactoSpore. We found that this combination has strong synergetic effects on phagocytosis and o n regulation of cholesterol and blood sugar levels. In addition, the Cinnulin/LastoSpore combination also reduced intestinal damage in mouse model of colitis .
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#1Vaclav Vetvicka (University of Louisville)H-Index: 28
#2Jana Vetvickova (University of Louisville)H-Index: 16
Perfluorinated compounds (PFCs) perfluoroctanic acid (PFOA) and perfluooctane sulphonic acid (PFOS) are environmentally widespread bioaccumulative chemicals known to induce profound effects on the immune system. In this study, two types of PFC, i.e., PFOS and PFOA, were administered orally (daily) a dose of 20 mg/kg/d. After exposure, all mice exhibited significant immunosuppressive effects upon both cellular (phagocytosis and NK cell activity) and humoral (antibody response) branches of their i...
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#1Vaclav Vetvicka (University of Louisville)H-Index: 28
#2Jana VetvickovaH-Index: 16
Last. S. FloydH-Index: 1
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Biological immunomodulators are routinely evaluated as a natural source of molecules with profound effects on the immune system. They belong to a group of physiologically active compounds, collectively termed biological response modifiers. Most of the studies were focused on immune system stimulation. Recently, they have become the focus of studies seeking molecules that are able to overcome negative effects of various immunotoxins. This paper concentrates on the effects of a glucan/resveratrol/...
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