Original paper
Targeted Gene Disruption by Homologous Recombination in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
Abstract
In contrast to the high accumulation in sequence data for hyperthermophilic archaea, methodology for genetically manipulating these strains is still at an early stage. This study aimed to develop a gene disruption system for the hyperthermophilic euryarchaeon Thermococcus kodakaraensis KOD1. Uracil-auxotrophic mutants with mutations in the orotidine-5′-monophosphate decarboxylase gene ( pyrF ) were isolated by positive selection using...
Paper Details
Title
Targeted Gene Disruption by Homologous Recombination in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
Published Date
Jan 1, 2003
Journal
Volume
185
Issue
1
Pages
210 - 220
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Notes
History