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In vitro effects of lithium and nickel at different levels on Neuro-2a mouse Neuroblastoma cells

Published on Aug 1, 2001in Toxicology in Vitro3.07
· DOI :10.1016/S0887-2333(01)00037-6
G. Repetto19
Estimated H-index: 19
,
A. del Peso6
Estimated H-index: 6
+ 1 AuthorsM. Repetto19
Estimated H-index: 19
Cite
Abstract
Lithium and nickel present low toxicity, but are able to cause alterations in different tissues. The toxic effects of lithium and nickel at different cellular levels were assessed using two inorganic chemical species: lithium chloride and nickel(II) chloride. Mouse neuroblastoma cell cultures (Neuro-2a) were exposed to both compounds for 24 h. The cytotoxic effects evaluated were cell proliferation by quantification of total protein content, cytoplasmic membrane integrity to cytosolic lactate dehydrogenase leakage, and lysosomal hexosaminidase release. Metabolic markers were lactate dehydrogenase activity and mitochondrial succinate dehydrogenase activity. Lysosomal markers were relative neutral red uptake by lysosomes, and lysosomal hexosaminidase sphingolipid degradation activity. Acetylcholinesterase activity on intact cells was also quantified. Nickel was found to be 36 times more toxic than lithium to neuroblastoma cell proliferation (EC50= 0.29 and 10.5 mm, respectively), but the relative extent of other alterations differed. Lithium stimulated nearly all the indicators studied, particularly lactate dehydrogenase, mitochondrial succinate dehydrogenase and acetylcholinesterase activities, as well as hexosaminidase release. In contrast, nickel mainly stimulated hexosaminidase release and inhibited lactate dehydrogenase activity. The stabilization of the cytoplasmic membrane to lactate dehydrogenase leakage simultaneously with the secretion of lysosomal hexosaminidase for both compounds also shows that functional metabolic alterations produced by lithium and nickel are more important than cytoplasmic damage. # 2001 Published by Elsevier Science Ltd.
  • References (22)
  • Citations (19)
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References22
Newest
Published on Jun 5, 1999in Journal of Biomedical Materials Research
John C. Wataha45
Estimated H-index: 45
(Georgia Regents University),
Petra E. Lockwood33
Estimated H-index: 33
(Georgia Regents University)
+ 1 AuthorsM. Ghazi1
Estimated H-index: 1
(GSU: Georgia State University)
Nickel-containing alloys commonly are used in medical and dental applications that place them into long-term contact with soft tissues. The release of Ni ions from these alloys is disturbing because of the toxic, immunologic, and carcinogenic effects that have been documented for some Ni compounds. In particular, Ni ions in solution recently have been shown to cause expression of inflammatory mediators, such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and intercellular adhesion m...
Published on Jan 1, 1999in Dental Materials4.44
R.L.W. Messer2
Estimated H-index: 2
(UAB: University of Alabama at Birmingham),
L. C. Lucas27
Estimated H-index: 27
(UAB: University of Alabama at Birmingham)
Abstract Objectives: Nickel-based dental alloys have been in use since 1930. However, there are concerns regarding the release of metal ions from these alloys to surrounding tissues. Cell culture evaluations can be used to address these concerns and to develop a biocompatibility model by providing a more basic understanding of the metabolic response to individual ions released from dental alloys. This study evaluates the metabolic as well as the morphological response of cultured human gingival ...
Published on Jan 1, 1999in Clinical Toxicology4.40
M. Rosario Repetto2
Estimated H-index: 2
,
M. Repetto19
Estimated H-index: 19
Background: The toxicological interpretation of blood and urine concentrations of drugs affecting the digestive system and metabolism, as with other groups of pharmaceutical specialities, is complicated. Methods: As a continuation of our published studies on the concentrations of drugs of abuse, drugs affecting cardiovascular and hematopoietic systems, and respiratory system, we have reviewed the published data relating to the concentrations of drugs affecting the digestive system and metabolism...
Published on Dec 1, 1996in Cell Biology International2.13
N.N. Hasgekar1
Estimated H-index: 1
,
P.P. Gokhale1
Estimated H-index: 1
(Sophia College, Mumbai)
+ 2 AuthorsV.S. Lalitha1
Estimated H-index: 1
Abstract The influence of lithium on cell growth and cell viability was studied in short-term cultures of a neural precursor cell line (NT) developed from a murine teratocarcinoma. At very low concentrations ranging from 0.1 m M to 1 m M Li 2 CO 3 (equivalent to therapeutic blood concentrations) there was no difference between untreated and treated cultures. 10 m M lithium (Li + ) was found to be toxic with 33% of cell death, while there was inhibition of growth without cell death at concentrati...
Published on Sep 1, 1994in Toxicology3.55
G. Repetto19
Estimated H-index: 19
,
P. Sanz10
Estimated H-index: 10
,
M. Repetto19
Estimated H-index: 19
Abstract The toxic effects of arsenic at different cellular levels were assessed using two inorganic chemical species: sodium arsenite and sodium arsenate, representing the trivalent and pentavalent states of arsenic, respectively. Mouse neuroblastoma cell cultures (Neuro-2a) were exposed for 24 h, and cytotoxic effects evaluated were: cell proliferation by quantification of total protein content; cytoplasmic membrane integrity to cytosolic lactate dehydrogenase leakage; lysosomal hexosaminidase...
Published on Jul 1, 1993in Toxicology in Vitro3.07
Erik Walum21
Estimated H-index: 21
(Stockholm University),
Marika K. Nordin4
Estimated H-index: 4
(Stockholm University)
+ 1 AuthorsL. Odland1
Estimated H-index: 1
(Stockholm University)
Abstract This review critically addresses key aspects of neurotoxicity that can be assessed by using in vitro test procedures and batteries. Such test schemes must most probably be hierarchical and multi-optional in order to be able to cope with the large number of possible mechanisms of neurotoxicity. Although the regenerative capacity of the nervous system is low, lesions can be compensated for by a number of cellular dynamic functions (e.g. intracellular calcium sequestration, membrane-bound ...
Published on Jul 1, 1993in Toxicology in Vitro3.07
Florianne Monnet-Tschudi26
Estimated H-index: 26
(UNIL: University of Lausanne),
Marie-Gabrielle Zurich23
Estimated H-index: 23
(UNIL: University of Lausanne),
Paul Honegger35
Estimated H-index: 35
(UNIL: University of Lausanne)
Abstract To evaluate their toxicity in the developing brain, eight metal compounds, [bismuth sodium tartrate (BiNA-tartrate), CdCl 2 , CoCl 2 , HgCl 2 , dimethyl mercury, NiCl 2 , TlCl and triethyltin chloride (TET)] were tested in aggregating cell cultures of foetal rat telencephalon. The compounds were applied to the cultures continuously, either during an early developmental stage (between days 5 and 14) or during and advanced stage of maturation (between days 24 and 34). Changes in the activ...
Cited By19
Newest
Published on Oct 24, 2018in Materials2.97
Teddy Tite1
Estimated H-index: 1
,
A.C. Popa7
Estimated H-index: 7
+ 4 AuthorsG.E. Stan17
Estimated H-index: 17
High-performance bioceramics are required for preventing failure and prolonging the life-time of bone grafting scaffolds and osseous implants. The proper identification and development of materials with extended functionalities addressing socio-economic needs and health problems constitute important and critical steps at the heart of clinical research. Recent findings in the realm of ion-substituted hydroxyapatite (HA) could pave the road towards significant developments in biomedicine, with an ...
Published on Sep 1, 2016in Neuroscience & Biobehavioral Reviews8.00
Giselli Scaini21
Estimated H-index: 21
(Unesc: Universidade do Extremo Sul Catarinense),
Gislaine T. Rezin22
Estimated H-index: 22
+ 3 AuthorsJoao L De Quevedo59
Estimated H-index: 59
Bipolar disorder (BD) is a chronic psychiatric illness characterized by severe and biphasic changes in mood. Several pathophysiological mechanisms have been hypothesized to underpin the neurobiology of BD, including the presence of mitochondrial dysfunction. A confluence of evidence points to an underlying dysfunction of mitochondria, including decreases in mitochondrial respiration, high-energy phosphates and pH; changes in mitochondrial morphology; increases in mitochondrial DNA polymorphisms;...
Published on Sep 1, 2015in Molecular Psychiatry11.97
Cagri Yuksel8
Estimated H-index: 8
,
Fei Du13
Estimated H-index: 13
+ 10 AuthorsSelma Sehovic3
Estimated H-index: 3
Abnormal high-energy phosphate molecule metabolism during regional brain activation in patients with bipolar disorder
Published on Mar 1, 2015in Neurotoxicology3.26
Claudia Ceci6
Estimated H-index: 6
(University of Rome Tor Vergata),
Maria Luisa Barbaccia27
Estimated H-index: 27
(University of Rome Tor Vergata),
Giuseppa Pistritto10
Estimated H-index: 10
(University of Rome Tor Vergata)
Abstract Nickel, a known occupational/environmental hazard, may cross the placenta and reach appreciable concentrations in various fetal organs, including the brain. The aim of this study was to investigate whether nickel interferes with the process of neuronal differentiation. Following a 4 week treatment with retinoic acid (10 μM), the human teratocarcinoma-derived NTera2/D1 cell line (NT2 cells) terminally differentiate into neurons which recapitulate many features of human fetal neurons. The...
Published on Dec 1, 2014in Polimeros-ciencia E Tecnologia0.81
Luiz Gabriel Maturana (UFVJM), Amauri Pierucci (UFVJM)+ 2 AuthorsEliana Aparecida de Rezende Duek18
Estimated H-index: 18
(State University of Campinas)
Resumo: Os biomateriais poli L-acido latico (PLLA) e o poli caprolactona (PCL) sao os polimeros mais estudadas na area dos materiais bioreabsorviveis. Dentre as suas principais caracteristicas que contribuem para a interacao celular, temos a especificidade quimica da superficie, eletrica, hidrofobicidade e topografia. Ainda, observa-se o tempo de degradacao, porosidade, biocompatibilidade com o tecido biologico, bem como, a confeccao com as mais variadas formas e dimensoes. Ja a pratica da cultu...
Published on Apr 1, 2014in Molecular and Cellular Biochemistry2.88
J. Chakraborty4
Estimated H-index: 4
(IICB: Indian Institute of Chemical Biology),
Usha Rajamma9
Estimated H-index: 9
,
Kochupurackal P. Mohanakumar30
Estimated H-index: 30
(IICB: Indian Institute of Chemical Biology)
Huntington’s disease (HD) is an autosomal dominant disease, with overt movement dysfunctions. Despite focused research on the basis of neurodegeneration in HD for last few decades, the mechanism for the site-specific lesion of neurons in the brain is not clear. All the explanations that partially clarify the phenomenon of neurodegeneration leads to one organelle, mitochondrion, which is severely affected in HD at the level of electron transport chain, Ca2+ buffering efficiency and morphology. Bu...
Published on Mar 12, 2013in Journal of the American Heart Association4.66
Mei Sun4
Estimated H-index: 4
(UHN: University Health Network),
Maral Ouzounian16
Estimated H-index: 16
(UHN: University Health Network)
+ 9 AuthorsAnthony O. Gramolini35
Estimated H-index: 35
(UHN: University Health Network)
Background Autophagy is critical in the maintenance of cellular protein quality control, the final step of which involves the fusion of autophagosomes with lysosomes. Cathepsin-L (CTSL) is a key member of the lysosomal protease family that is expressed in the murine and human heart, and it may play an important role in protein turnover. We hypothesized that CTSL is important in regulating protein processing in the heart, particularly under pathological stress. Methods and Results Phenylephrine-i...
Published on Mar 1, 2013in Archives of Oral Biology1.66
Symone San Miguel7
Estimated H-index: 7
(Texas A&M Health Science Center),
Lynne A. Opperman31
Estimated H-index: 31
(Texas A&M Health Science Center)
+ 2 AuthorsKathy K.H. Svoboda24
Estimated H-index: 24
(Texas A&M Health Science Center)
Abstract Objective In dentistry, the use of metals in fillings, braces, implants, bridges and other prosthodontic restorations is a common practice. Previous studies revealed that zinc (Zn) and copper (Cu) released from gold alloys, and nickel (Ni) released from nickel–chromium alloys, have a highly cytotoxic effect on fibroblast cell cultures. Our working hypothesis is that oral fibroblasts are susceptible to damage from metals because they elevate reaction oxygen species (ROS). In this study, ...
Published on May 1, 2012in Journal of The Chinese Chemical Society1.19
Muhammad Akhyar Farrukh15
Estimated H-index: 15
(Government College University),
Sana Majeed1
Estimated H-index: 1
(Government College University)
Oxidation of sodium succinate in aqueous solution by potassium ferricyanide was investigated. The effects of different reaction parameters such as initial concentration of succinate, concentration of ferricyanide, and influence of metal ions (Zn+2, Ni+2, Cu+2, Cd+2) on the oxidation of succinate were investigated at 25 ± 0.05 °C. Large rate enhancements were observed in the redox reaction between succinate and ferricyanide with increasing concentrations of both oxidant and reductant. The kinetic...
Published on Mar 8, 2012in Sensors3.03
Rebekka Kubisch2
Estimated H-index: 2
,
Ulrich Bohrn5
Estimated H-index: 5
+ 1 AuthorsEvamaria Stütz6
Estimated H-index: 6
Pollution of drinking water sources represents a continuously emerging problem in global environmental protection. Novel techniques for real-time monitoring of water quality, capable of the detection of unanticipated toxic and bioactive substances, are urgently needed. In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown. Readout parameters of the cells were the acidification (metabolism), oxygen cons...
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