Determination of a novel phosphodiesterase-4 inhibitor chlorbipram in mouse plasma and brain by UFLC–MS/MS: Application in pharmacokinetic studies after intravenous administration

In this study, we evaluated a simple and sensitive method for determination of a novel phosphodiesterase-4 (PDE4) inhibitor, chlorbipram, in mouse plasma and brain using ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS). Separation was achieved using an Acquity UPLC BEH C18 column (50mm×2.1mm, particle size 1.7μm) with a gradient mobile phase consisting of water and methanol at a flow rate of 0.25ml/min. Detection was...