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Avian Metapneumovirus Subtypes Circulating in Brazilian Vaccinated and Nonvaccinated Chicken and Turkey Farms

Published on Mar 1, 2011in Avian Diseases1.306
· DOI :10.1637/9501-081310-Reg.1
Jorge Luis Chacón9
Estimated H-index: 9
,
Matheus Y. Mizuma2
Estimated H-index: 2
+ 5 AuthorsAntonio José Piantino Ferreira24
Estimated H-index: 24
Abstract
SUMMARY. Avian metapneumovirus (AMPV) causes turkey rhinotracheitis and is associated with swollen head syndrome in chickens, which is usually accompanied by secondary infections that increase mortality. AMPVs circulating in Brazilian vaccinated and nonvaccinated commercial chicken and turkey farms were detected using a universal reverse transcriptase (RT)-PCR assay that can detect the four recognized subtypes of AMPV. The AMPV status of 228 farms with respiratory and reproductive disturbances was investigated. AMPV was detected in broiler, hen, breeder, and turkey farms from six different geographic regions of Brazil. The detected viruses were subtyped using a nested RT-PCR assay and sequence analysis of the G gene. Only subtypes A and B were detected in both vaccinated and nonvaccinated farms. AMPV-A and AMPV-B were detected in 15 and 23 farms, respectively, while both subtypes were simultaneously found in one hen farm. Both vaccine and field viruses were detected in nonvaccinated farms. In five cases, ...
  • References (36)
  • Citations (9)
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References36
Newest
#1Elena Catelli (UNIBO: University of Bologna)H-Index: 13
#2C. Lupini (UNIBO: University of Bologna)H-Index: 3
Last. Clive J. Naylor (University of Liverpool)H-Index: 18
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Live avian metapneumovirus (AMPV) vaccines have largely brought turkey rhinotracheitis (TRT) under control in Europe but unexplained outbreaks still occur. Italian AMPV longitudinal farm studies showed that subtype B AMPVs were frequently detected in turkeys some considerable period after subtype B vaccination. Sequencing showed these to be unrelated to the previously applied vaccine. Sequencing of the entire genome of a typical later isolate showed numerous SH and G protein gene differences whe...
37 CitationsSource
The avian metapneumovirus (aMPV) is the causative agent of an acute respiratory disease in turkeys, which causes considerable economic losses to the poultry industry. Currently attenuated live and inactivated vaccines are widely used to control the disease, but vaccine breaks are frequently observed. For improvement of current vaccination strategies it is necessary to gain enhanced knowledge of the immune mechanisms against aMPV infection. Field observations suggest that vaccine-induced aMPV-spe...
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#1Caroline Banet-Noach (TAU: Tel Aviv University)H-Index: 14
#2Lubov SimanovH-Index: 9
Last. Eran Bacharach (TAU: Tel Aviv University)H-Index: 23
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Abstract Avian metapneumovirus (aMPV) is a respiratory pathogen and includes four subtypes, which are differentially distributed worldwide. In Israel, two aMPV subtypes, A and B, are cocirculating in an area with a relatively high density of poultry houses. We performed a longitudinal survey in commercial flocks of turkeys and chickens (six and two flocks, respectively) with differing vaccination programs. This study revealed that all monitored flocks were infected by aMPV during the sampling pe...
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#1M. CecchinatoH-Index: 1
#2Elena CatelliH-Index: 13
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#1Jorge Luis Chacón (USP: University of São Paulo)H-Index: 9
#2Paulo Eduardo Brandão (USP: University of São Paulo)H-Index: 18
Last. Antonio José Piantino Ferreira (USP: University of São Paulo)H-Index: 24
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Subtype B avian metapneumovirus (aMPV) was isolated and detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in Brazilian commercial laying chicken flocks with no history of vaccination against aMPV and presenting respiratory signs and decreased egg production. RT-PCR results from samples from three affected flocks revealed that the three isolates were subtype B. Partial sequence analysis of the G glycoprotein gene confirmed that the samples belonged to subtype B and were not of ...
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#1Kannan Ganapathy (University of Liverpool)H-Index: 13
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Abstract Live attenuated subtype B avian metapneumovirus (aMPV) vaccines from two different commercial sources (Vac 1 and Vac 2) were used to vaccinate two groups of day-old specific-pathogen-free chicks. The chicks were challenged at 21 or 49 days of age with a virulent subtype B virus. Parameters compared were persistence of vaccine viruses, their ability to induce humoral antibody responses, and the protection they offered against virulent challenge. Vac 1 virus was detectable for at least 14...
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#1Eun Ho Lee (CBNU: Chungbuk National University)H-Index: 5
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Abstract Complete nucleotide sequences of two avian metapneumoviruses (aMPV), designated PL-1 and PL-2, were isolated from pheasants, revealing novel sequences of the first aMPV to be fully sequenced in Korea. The complete genome of both PL-1 and PL-2 was composed of 13,170 nucleotides. Phylogenetic analysis revealed that PL-1 belonged to aMPV subtype C, sharing higher homology in deduced amino acid sequence identities with hMPV, rather than with aMPV subtypes A and B. Replication of PL-1 in exp...
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#2Cecchinato Mattia (UNIBO: University of Bologna)H-Index: 6
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A live A type avian metapneumovirus (AMPV) vaccine which had been shown to be highly protective and short lived in experimental conditions was found to persist for longer periods in the field and to be associated with disease. Previously other factors such as possible secondary pathogens and management considerations had made it impossible to conclude whether the observed disease was a result of an increase in the vaccine virulence. In this study, an AMPV was isolated from poults on a farm which...
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#1Miki Sugiyama (Merial)H-Index: 2
#2Hiroyuki Koimaru (Merial)H-Index: 1
Last. Toshihiro Ito (Tottori University)H-Index: 25
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Decreases in egg production and increased incidence of abnormal eggs due to malformation of egg shells were observed in specific pathogen free (SPF) 173-day-old laying hens inoculated intravenously with an avian metapneumovirus (aMPV) strain PLE8T1. This strain was derived from an isolate from broiler birds exhibiting swollen head syndrome (SHS). Some SPF birds inoculated with the virus showed, slight diarrhea without any respiratory symptoms. Thus, the PLE8T1 strain was used as a challenge viru...
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Although the disease associated with the avian metapneumovirus (aMPV) has been described in Israel for more than 20 years and one Israeli isolate was characterized as subtype A, this is the first study investigating the strains circulating in Israel (period 2002 to 2004). Viral RNA was detected by reverse transcription-polymerase chain reaction in 44% of the examined flocks, and one-half of the virus isolation attempts were successful. Characterization showed that 34 non-vaccinated turkey flocks...
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Live vaccines predominantly control avian metapneumovirus (aMPV) infection in poultry flocks, but vaccine virus can be found for extended periods after application. The most frequently used aMPV vaccine in Italy, VCO3 subtype B, was shown to contain a unique Tru9I restriction endonuclease site within the amplicons produced by a commonly used aMPV diagnostic reverse transcriptase (RT)-nested polymerase chain reaction (PCR). Analysis of European and database logged subtype B aMPV sequences confirm...
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Avian metapneumovirus (aMPV) is a respiratory pathogen associated with the swollen head syndrome (SHS) in chickens. In Brazil, live aMPV vaccines are currently used, but subtypes A and, mainly subtype B (aMPV/A and aMPV/B) are still circulating. This study was conducted to characterize two Brazilian aMPV isolates (A and B subtypes) of chicken origin. A challenge trial to explore the replication ability of the Brazilian subtypes A and B in chickens was performed. Subsequently, virological protect...
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