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Avian Metapneumovirus Subtypes Circulating in Brazilian Vaccinated and Nonvaccinated Chicken and Turkey Farms

Published on Mar 1, 2011in Avian Diseases1.31
· DOI :10.1637/9501-081310-Reg.1
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo),
Matheus Y. Mizuma2
Estimated H-index: 2
(USP: University of São Paulo)
+ 5 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Cite
Abstract
SUMMARY. Avian metapneumovirus (AMPV) causes turkey rhinotracheitis and is associated with swollen head syndrome in chickens, which is usually accompanied by secondary infections that increase mortality. AMPVs circulating in Brazilian vaccinated and nonvaccinated commercial chicken and turkey farms were detected using a universal reverse transcriptase (RT)-PCR assay that can detect the four recognized subtypes of AMPV. The AMPV status of 228 farms with respiratory and reproductive disturbances was investigated. AMPV was detected in broiler, hen, breeder, and turkey farms from six different geographic regions of Brazil. The detected viruses were subtyped using a nested RT-PCR assay and sequence analysis of the G gene. Only subtypes A and B were detected in both vaccinated and nonvaccinated farms. AMPV-A and AMPV-B were detected in 15 and 23 farms, respectively, while both subtypes were simultaneously found in one hen farm. Both vaccine and field viruses were detected in nonvaccinated farms. In five cases, ...
  • References (36)
  • Citations (9)
Cite
References36
Newest
Published on Jan 1, 2010in Vaccine3.27
E. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna),
C. Lupini3
Estimated H-index: 3
(UNIBO: University of Bologna)
+ 3 AuthorsC. J. Naylor17
Estimated H-index: 17
(University of Liverpool)
Live avian metapneumovirus (AMPV) vaccines have largely brought turkey rhinotracheitis (TRT) under control in Europe but unexplained outbreaks still occur. Italian AMPV longitudinal farm studies showed that subtype B AMPVs were frequently detected in turkeys some considerable period after subtype B vaccination. Sequencing showed these to be unrelated to the previously applied vaccine. Sequencing of the entire genome of a typical later isolate showed numerous SH and G protein gene differences whe...
Published on Dec 1, 2009in Avian Pathology1.96
Dennis Rubbenstroth16
Estimated H-index: 16
,
Silke Rautenschlein20
Estimated H-index: 20
The avian metapneumovirus (aMPV) is the causative agent of an acute respiratory disease in turkeys, which causes considerable economic losses to the poultry industry. Currently attenuated live and inactivated vaccines are widely used to control the disease, but vaccine breaks are frequently observed. For improvement of current vaccination strategies it is necessary to gain enhanced knowledge of the immune mechanisms against aMPV infection. Field observations suggest that vaccine-induced aMPV-spe...
Published on Jun 1, 2009in Avian Diseases1.31
Caroline Banet-Noach14
Estimated H-index: 14
(TAU: Tel Aviv University),
Lubov Simanov9
Estimated H-index: 9
+ 2 AuthorsEran Bacharach22
Estimated H-index: 22
(TAU: Tel Aviv University)
Abstract Avian metapneumovirus (aMPV) is a respiratory pathogen and includes four subtypes, which are differentially distributed worldwide. In Israel, two aMPV subtypes, A and B, are cocirculating in an area with a relatively high density of poultry houses. We performed a longitudinal survey in commercial flocks of turkeys and chickens (six and two flocks, respectively) with differing vaccination programs. This study revealed that all monitored flocks were infected by aMPV during the sampling pe...
Published on Oct 1, 2007in Avian Pathology1.96
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo),
Paulo Eduardo Brandão18
Estimated H-index: 18
(USP: University of São Paulo)
+ 2 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Subtype B avian metapneumovirus (aMPV) was isolated and detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in Brazilian commercial laying chicken flocks with no history of vaccination against aMPV and presenting respiratory signs and decreased egg production. RT-PCR results from samples from three affected flocks revealed that the three isolates were subtype B. Partial sequence analysis of the G glycoprotein gene confirmed that the samples belonged to subtype B and were not of ...
Published on Sep 1, 2007in Virus Research2.74
Eun Ho Lee5
Estimated H-index: 5
(CBNU: Chungbuk National University),
Min-Suk Song24
Estimated H-index: 24
(CBNU: Chungbuk National University)
+ 5 AuthorsYoung Ki Choi29
Estimated H-index: 29
(CBNU: Chungbuk National University)
Abstract Complete nucleotide sequences of two avian metapneumoviruses (aMPV), designated PL-1 and PL-2, were isolated from pheasants, revealing novel sequences of the first aMPV to be fully sequenced in Korea. The complete genome of both PL-1 and PL-2 was composed of 13,170 nucleotides. Phylogenetic analysis revealed that PL-1 belonged to aMPV subtype C, sharing higher homology in deduced amino acid sequence identities with hMPV, rather than with aMPV subtypes A and B. Replication of PL-1 in exp...
Published on Sep 1, 2007in Avian Diseases1.31
Kannan Ganapathy12
Estimated H-index: 12
,
R. C. Jones35
Estimated H-index: 35
Abstract Live attenuated subtype B avian metapneumovirus (aMPV) vaccines from two different commercial sources (Vac 1 and Vac 2) were used to vaccinate two groups of day-old specific-pathogen-free chicks. The chicks were challenged at 21 or 49 days of age with a virulent subtype B virus. Parameters compared were persistence of vaccine viruses, their ability to induce humoral antibody responses, and the protection they offered against virulent challenge. Vac 1 virus was detectable for at least 14...
Published on Oct 1, 2006in Vaccine3.27
E. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna),
Cecchinato Mattia6
Estimated H-index: 6
(UNIBO: University of Bologna)
+ 2 AuthorsC. J. Naylor17
Estimated H-index: 17
(University of Liverpool)
A live A type avian metapneumovirus (AMPV) vaccine which had been shown to be highly protective and short lived in experimental conditions was found to persist for longer periods in the field and to be associated with disease. Previously other factors such as possible secondary pathogens and management considerations had made it impossible to conclude whether the observed disease was a result of an increase in the vaccine virulence. In this study, an AMPV was isolated from poults on a farm which...
Published on Jan 1, 2006in Journal of Veterinary Medical Science0.91
Miki Sugiyama2
Estimated H-index: 2
(Merial),
Hiroyuki Koimaru1
Estimated H-index: 1
(Merial)
+ 3 AuthorsToshihiro Ito25
Estimated H-index: 25
(Tottori University)
Decreases in egg production and increased incidence of abnormal eggs due to malformation of egg shells were observed in specific pathogen free (SPF) 173-day-old laying hens inoculated intravenously with an avian metapneumovirus (aMPV) strain PLE8T1. This strain was derived from an isolate from broiler birds exhibiting swollen head syndrome (SHS). Some SPF birds inoculated with the virus showed, slight diarrhea without any respiratory symptoms. Thus, the PLE8T1 strain was used as a challenge viru...
Published on Jun 1, 2005in Avian Pathology1.96
Caroline Banet-Noach14
Estimated H-index: 14
,
Lubov Simanov9
Estimated H-index: 9
,
Shimon Perk9
Estimated H-index: 9
Although the disease associated with the avian metapneumovirus (aMPV) has been described in Israel for more than 20 years and one Israeli isolate was characterized as subtype A, this is the first study investigating the strains circulating in Israel (period 2002 to 2004). Viral RNA was detected by reverse transcription-polymerase chain reaction in 44% of the examined flocks, and one-half of the virus isolation attempts were successful. Characterization showed that 34 non-vaccinated turkey flocks...
Cited By9
Newest
Published on Sep 1, 2018in Virus Research2.74
Zongxi Han21
Estimated H-index: 21
(Harbin Veterinary Research Institute),
Mengying Gao5
Estimated H-index: 5
(Harbin Veterinary Research Institute)
+ 4 AuthorsShengwang Liu22
Estimated H-index: 22
(Harbin Veterinary Research Institute)
Abstract Three different IBV genotypes/serotypes, designated ck/CH/LDL/150434–I (LDL/150434–I), ck/CH/LDL/150434–II (LDL/150434–II) and ck/CH/LDL/150434–III (LDL/150434–III), were detected in a single tracheal sample from a chicken showing signs of respiratory disease. The viruses were isolated using a cross-neutralization test and limiting dilution in embryonated specific-pathogen-free (SPF) eggs. Isolate LDL/150434–I was a re-isolation of H120 vaccine strain that was introduced into the chicke...
Published on Aug 1, 2014in Tropical Animal Health and Production1.09
Abdel-Azeem Sayed Abdel-Azeem1
Estimated H-index: 1
(FU: Fayoum University),
Giovanni Franzo10
Estimated H-index: 10
(UNIPD: University of Padua)
+ 5 AuthorsMattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua)
Although avian metapneumovirus (aMPV) infection has been reported in most regions of the world, to date, only subtype B has been detected in Egypt. At the end of November 2013, dry oropharyngeal swabs were collected during an outbreak of respiratory diseases in a free-range, multi-age turkey dealer farm in Northern Upper Egypt. The clinical signs that appeared when turkeys were 3 weeks-old were characterized by ocular and nasal discharge and swelling of sinuses. aMPV of subtype A was detected by...
Published on Jan 2, 2014in Avian Pathology1.96
V. Listorti4
Estimated H-index: 4
(UNIBO: University of Bologna),
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna)
+ 5 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
Live vaccines predominantly control avian metapneumovirus (aMPV) infection in poultry flocks, but vaccine virus can be found for extended periods after application. The most frequently used aMPV vaccine in Italy, VCO3 subtype B, was shown to contain a unique Tru9I restriction endonuclease site within the amplicons produced by a commonly used aMPV diagnostic reverse transcriptase (RT)-nested polymerase chain reaction (PCR). Analysis of European and database logged subtype B aMPV sequences confirm...
Published on Jun 1, 2013in Avian Pathology1.96
Mattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua),
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna)
+ 4 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
In recent years, special attention has been paid to real-time polymerase chain reaction (PCR) for avian metapneumovirus (AMPV) diagnosis, due to its numerous advantages over classical PCR. A new multiplex quantitative real-time reverse transcription-PCR (qRT-PCR) with molecular beacon probe assay, designed to target the SH gene, was developed. The test was evaluated in terms of specificity, sensitivity and repeatability, and compared with conventional RT nested-PCR based on the G gene. All of th...
Published on Jan 1, 2013in Virology Journal2.46
Ra Cha2
Estimated H-index: 2
(USDA: United States Department of Agriculture),
Qingzhong Yu13
Estimated H-index: 13
(USDA: United States Department of Agriculture),
L. Zsak28
Estimated H-index: 28
(USDA: United States Department of Agriculture)
Background Avian metapneumovirus subtype C (aMPV/C) causes severe upper respiratory disease in turkeys. Previous report revealed the presence of aMPV/C in wild birds in the southeast regions of the U.S.
Published on Jan 1, 2013in World Journal of Vaccines
Qingzhong Yu13
Estimated H-index: 13
(USDA: United States Department of Agriculture),
Jason P. Roth3
Estimated H-index: 3
+ 3 AuthorsL. Zsak28
Estimated H-index: 28
Avian metapneumovirus (aMPV) and Newcastle disease virus (NDV) are threatening avian pathogens that can cause serious respiratory diseases in poultry worldwide. Vaccination, combined with strict biosecurity practices, has been the recommendation for controlling these diseases in the field. In the present study, we generated NDV LaSota vaccine strain-based recombinant viruses expressing the glycoprotein (G) of aMPV, subtype A or B, using reverse genetics technology. These recombinant viruses, rLS...
Published on Dec 1, 2012in Pesquisa Veterinaria Brasileira0.30
Márcia Bianchi dos Santos3
Estimated H-index: 3
(State University of Campinas),
Matheus C. Martini4
Estimated H-index: 4
(State University of Campinas)
+ 4 AuthorsClarice Weis Arns16
Estimated H-index: 16
(State University of Campinas)
Avian metapneumovirus (aMPV) is a respiratory pathogen associated with the swollen head syndrome (SHS) in chickens. In Brazil, live aMPV vaccines are currently used, but subtypes A and, mainly subtype B (aMPV/A and aMPV/B) are still circulating. This study was conducted to characterize two Brazilian aMPV isolates (A and B subtypes) of chicken origin. A challenge trial to explore the replication ability of the Brazilian subtypes A and B in chickens was performed. Subsequently, virological protect...
Published on Dec 1, 2012in Avian Diseases1.31
Mattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua),
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna)
+ 4 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
SUMMARY. The current information on the prevalence of avian metapneumovirus (aMPV) infection in layers is fragmentary and its true impact on egg production often remains unknown or unclear. In order to draw an epidemiologic picture of aMPV presence in layer flocks in Italy, a survey was performed on 19 flocks of pullets and layers based on longitudinal studies or sporadic samplings. aMPV was detected by reverse transcription (RT)-PCR, and blood samples were collected for serology by aMPV ELISA. ...
Published on Nov 1, 2011in Vaccine3.27
Haixia Hu3
Estimated H-index: 3
(JLU: Jilin University),
Jason P. Roth3
Estimated H-index: 3
(USDA: United States Department of Agriculture)
+ 3 AuthorsQingzhong Yu13
Estimated H-index: 13
(USDA: United States Department of Agriculture)
a b s t r a c t Virulent strains of Newcastle disease virus (NDV) and avian metapneumovirus (aMPV) can cause seri- ous respiratory diseases in poultry. Vaccination combined with strict biosecurity practices has been the recommendation for controlling both NDV and aMPV diseases in the field. In the present study, an NDV based, LaSota strain recombinant vaccine virus expressing the glycoprotein (G) of aMPV subgroup C (aMPV-C) was generated as a bivalent vaccine using a reverse genetics approach. T...