Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Abstract
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in...
Paper Details
Title
Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Published Date
Jan 1, 2013
Journal
Volume
194
Issue
4
Pages
1029 - 1035
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