Detection of human neutrophil elastase with peptide-bound cross-linked ethoxylate acrylate resin analogs

: An assessment of elastase‐substrate kinetics and adsorption at the solid–liquid interface of peptide‐bound resin was made in an approach to the solid‐phase detection of human neutrophil elastase (HNE), which is found in high concentration in chronic wound fluid. N‐succinyl‐alanine‐alanine‐proline‐valine‐ p ‐nitroanilide (suc‐Ala‐Ala‐Pro‐Val‐ p NA), a chromogenic HNE substrate, was attached to glycine‐cross‐linked ethoxylate acrylate resins...