Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source

Although glucose can be used as an economical and efficient energy source for driving cell-free protein synthesis, use of high concentrations of glucose can cause accumulation of organic acids, which inhibits protein synthesis through the decrease in pH of reaction mixture. In this study, instead of directly using glucose, we used soluble starch as a glucose-releasing energy reservoir molecule. When compared to previously reported methods using...