Original paper
Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source
Abstract
Although glucose can be used as an economical and efficient energy source for driving cell-free protein synthesis, use of high concentrations of glucose can cause accumulation of organic acids, which inhibits protein synthesis through the decrease in pH of reaction mixture. In this study, instead of directly using glucose, we used soluble starch as a glucose-releasing energy reservoir molecule. When compared to previously reported methods using...
Paper Details
Title
Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source
Published Date
Jun 1, 2011
Journal
Volume
46
Issue
6
Pages
1366 - 1369
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Notes
History