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Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells

Published on Jun 1, 2011in Journal of Ethnopharmacology 3.41
· DOI :10.1016/j.jep.2011.04.060
Hye Jin Kim9
Estimated H-index: 9
(CNU: Chungnam National University),
Jin Chul Kim5
Estimated H-index: 5
(CNU: Chungnam National University)
+ 5 AuthorsJeong Keun Ahn7
Estimated H-index: 7
(CNU: Chungnam National University)
Cite
Abstract
Abstract Ethnopharmacological relevance A medicinal herb Tribulus terrestris Linn has been used to treat various diseases including hepatocellular carcinoma. The aim of the present study was to investigate the anticancer activity of Tribulus terrestris Linn (TT) in liver cancer cells. Materials and methods The antitumor activity of aqueous TT extract was analyzed by testing the cytotoxicity and the effect on clonogenecity in HepG2 cells. Apoptosis and cell cycle arrest induced by TT were dissected by flow cytometry and its inhibitory effect on NF-κB activity was determined by analyzing the expression levels of NF-κB/IκB subunit proteins. The suppression of NF-κB-regulated gene expression by TT was assessed by RT-PCR. Results TT extract repressed clonogenecity and proliferation, induced apoptosis, and enhanced accumulation in the G0/G1 phase of liver cancer cells. It also turned out that TT extract inhibited NF-κB-dependent reporter gene expression and NF-κB subunit p50 expression, while it enhanced the cellular level of IκBα by inhibiting the phosphorylation and degradation of IκBα. In addition, IKK activity was inhibited in a dose-dependent manner. Furthermore, TT extract suppressed the transcription of genes associated with cell cycle regulation, anti-apoptosis, and invasion. Conclusion These data showed that TT extract blocks proliferation and induces apoptosis in human liver cancer cells through the inhibition of NF-κB signaling. Aqueous TT extract can be used as an anticancer drug for hepatocellular carcinoma patients.
  • References (24)
  • Citations (29)
Cite
References24
Newest
Published on Jul 1, 2009in Journal of Ethnopharmacology 3.41
Hye Jin Kim9
Estimated H-index: 9
(CNU: Chungnam National University),
Hwa Seung Yoo1
Estimated H-index: 1
(Daejeon University)
+ 8 AuthorsSeong Woo Yoon6
Estimated H-index: 6
(Kyung Hee University)
Abstract Ethnopharmacological relevance A medicinal herb Curcuma longa Linn has been used for treating various liver diseases caused by hepatitis B virus (HBV) in Asia. Aim of the study The study was performed in order to investigate the antiviral activity of Curcuma longa Linn against HBV replication in liver cells. Materials and methods Aqueous extract of Curcuma longa Linn (CLL) was prepared and used to analyze its antiviral activity against HBV replication in HepG 2.2.15 cells, which contain...
Published on Mar 1, 2008in Cell Research 17.85
Michael Karin208
Estimated H-index: 208
Published on Mar 1, 2008in Cell Research 17.85
Michael Karin208
Estimated H-index: 208
A potential link between inflammation and cancer has been suspected for over a century, but the exact molecular mechanisms connecting the two remained nebulous. We proposed that NF-kappaB transcription factors regulated via the IkappaB kinase (IKK) complex play a critical role in coupling inflammation and cancer and have set out to test this hypothesis in mouse models of cancer. Using mice bearing mutations in the genes coding for the IKKbeta and IKKalpha catalytic subunits we obtained evidence ...
Published on May 15, 2007in International Journal of Cancer 4.98
Taeko Okudaira15
Estimated H-index: 15
(University of the Ryukyus),
Mitsuomi Hirashima30
Estimated H-index: 30
(Kagawa University)
+ 9 AuthorsMasato Masuda26
Estimated H-index: 26
(University of the Ryukyus)
Retraction: The following article has been retracted through agreement between the first author and several coauthors, the journal Editor-in-Chief, Peter Lichter, and John Wiley & Sons, Ltd.: Okudaira T, Hirashima M, Ishikawa C, Makishi S, Tomita M, Matsuda T, Kawakami H, Taira N, Ohshiro K, Masuda M, Takasu N, Mori N. (2007). A modified version of galectin-9 suppresses cell growth and induces apoptosis of human T-cell leukemia virus type I-infected T-cell lines. Int J Cancer; 120 (May (10)): 22...
Published on Feb 1, 2007in Current Pharmaceutical Design 2.41
Takashi Okamoto39
Estimated H-index: 39
,
Takaomi Sanda28
Estimated H-index: 28
,
Kaori Asamitsu19
Estimated H-index: 19
NF-κB is an inducible transcription factor that is controlled by the signal activation cascades. NF-κB controls a number of genes involved in immuno-inflammatory responses, cell cycle progression, inhibition of apoptosis and cell adhesion, thus promoting carcinogenesis and cancer progression. Interestingly, some proteins encoded by oncogenes and oncogenic viruses have been shown to be involved in NF-κB activation pathway. In fact, NF-κB is constitutively activated in some cancer and leukemia cel...
Published on Jan 1, 2007in Zeitschrift für Naturforschung C
El-Sayed S. Abdel-Hameed8
Estimated H-index: 8
,
Hanan A. El-Nahas4
Estimated H-index: 4
,
Eman A. El-Wakil6
Estimated H-index: 6
The methanol extract of the whole parts of Tribulus macropterus Boiss. (family Zygophyllaceae) showed cytotoxic activity against a human tumour cell line (hepatocyte generation 2, HepG2) (IC50 = 2.9 μg/ml). The n-butanolic fraction obtained from successive fractionation of the methanolic extract exhibited activity against HepG2 (IC50 = 2.6 μg/ml). Therefore, this fraction was subjected to separation using different chromatographic techniques. Five compounds, 1Ð5, were isolated and identified as:...
Published on Dec 1, 2006in Nature Protocols 11.33
Nicolaas A. P. Franken21
Estimated H-index: 21
(UvA: University of Amsterdam),
H. M. Rozermond18
Estimated H-index: 18
(UvA: University of Amsterdam)
+ 2 AuthorsC. van Bree18
Estimated H-index: 18
(UvA: University of Amsterdam)
Clonogenic assay or colony formation assay is an in vitro cell survival assay based on the ability of a single cell to grow into a colony. The colony is defined to consist of at least 50 cells. The assay essentially tests every cell in the population for its ability to undergo “unlimited” division. Clonogenic assay is the method of choice to determine cell reproductive death after treatment with ionizing radiation, but can also be used to determine the effectiveness of other cytotoxic agents. On...
Published on Nov 1, 2006in Annals of the New York Academy of Sciences 4.29
Amr Amin18
Estimated H-index: 18
(United Arab Emirates University),
Mohamed Lotfy6
Estimated H-index: 6
(United Arab Emirates University)
+ 1 AuthorsErnest Adeghate25
Estimated H-index: 25
(United Arab Emirates University)
Abstract: Tribulus terrestris L (TT) is used in the Arabic folk medicine to treat various diseases. The aim of this article was to investigate the protective effects of TT in diabetes mellitus (DM). Diabetes is known to increase reactive oxygen species (ROS) level that subsequently contributes to the pathogenesis of diabetes. Rats were divided into six groups and treated with either saline, glibenclamide (Glib), or TT for 30 days. Rats in group 1 were given saline after the onset of streptozotoc...
Published on Aug 7, 2006in Journal of Experimental Medicine 10.89
Anjli Kukreja8
Estimated H-index: 8
,
Aisha Hutchinson3
Estimated H-index: 3
+ 5 AuthorsMadhav V. Dhodapkar56
Estimated H-index: 56
Infiltration by dendritic cells (DCs) is a common feature of most human tumors. Prior studies evaluating the interaction of DCs with tumors have focused largely on their immunologic properties (for review see Banchereau, J., and R.M. Steinman. 1998. Nature . 392:245–252). In this study, we show that the clonogenicity of several human tumor cell lines and primary tumor cells from myeloma patients is enhanced by their interactions with DCs. Myeloma cells cultured in the presence of DCs have an alt...
Published on Apr 1, 2006in Journal of Ethnopharmacology 3.41
Oludotun A. Phillips15
Estimated H-index: 15
(Kuniv: Kuwait University),
Koyippalli T. Mathew1
Estimated H-index: 1
(Kuniv: Kuwait University),
Mabayoje A. Oriowo14
Estimated H-index: 14
(Kuniv: Kuwait University)
Abstract The effects of methanolic and aqueous extracts of Tribulus terrestris on rat blood pressure (BP) and the perfused mesenteric vascular bed were investigated. The extracts dose-dependently reduced BP in spontaneously hypertensive rats (SHRs) with the aqueous fraction being more potent than the methanolic fraction at all doses tested. In vitro, the methanolic but not aqueous extract produced a dose-dependent increase in perfusion pressure of the mesenteric vascular bed. When perfusion pres...
Cited By29
Newest
Published on Mar 7, 2019in Journal of Cellular Physiology 4.52
Abdolmajid Ghasemian5
Estimated H-index: 5
(IAU: Islamic Azad University),
Abdolmajid Ghasemian1
Estimated H-index: 1
(IAU: Islamic Azad University)
+ 8 AuthorsNeda Sadat Shokouhi Mostafavi (ZNU: University of Zanjan)
Published in Heliyon
Chunlian Tian (Yantai University), yu chang (Shenyang Agricultural University)+ -3 AuthorsMingchun Liu3
Estimated H-index: 3
(Shenyang Agricultural University)
Abstract Research on the extraction technology, component analysis, and antioxidant, antibacterial, analgesic and anti-inflammatory activities of flavonoids fraction from Tribulus terrestris L. leaves was carried out. The extraction process was optimized by response surface method, and the maximum yield 0.27% for flavonoids fraction was achieved with concentration of ethanol solution 25.87%, ratio of solvent to material 30:1 ml/g, and ultrasonic extraction time 27.93 min. Moreover, 14 compositio...
Published on Feb 1, 2019
Apurva Patel (Veer Narmad South Gujarat University), Anjali Soni1
Estimated H-index: 1
(Veer Narmad South Gujarat University)
+ 1 AuthorsPreeti Sharma1
Estimated H-index: 1
(Veer Narmad South Gujarat University)
Tribulus terrestris (TT), a herb belonging to Zygophyllaceae family is widely used due to its medicinal properties. This study was undertaken to elucidate the anticancer mechanism of TT on MCF-7 breast cancer cells. Cytotoxic effect of the herb was assessed by 3-(4,5-diethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Apoptotic potential was assessed through DNA fragmentation, TUNEL and caspase 3 activity assays. Expressions of genes regulating the apoptotic pathway were examined ...
Published on Feb 1, 2019in Complementary Therapies in Medicine 1.98
Sima Sanagoo1
Estimated H-index: 1
(Tabriz University of Medical Sciences),
Behnaz Sadeghzadeh Oskouei1
Estimated H-index: 1
(Tabriz University of Medical Sciences)
+ 3 AuthorsAzizeh Farshbaf-Khalili5
Estimated H-index: 5
(Tabriz University of Medical Sciences)
Abstract Background and objective(S) Tribulus terrestris L. is an herbal plant that has long been used as sex stimulant and to treat male infertility. This systematic review collected the clinical trials and/or quasi-experimental studies on the effect of T. terrestris on sperm parameters in idiopathic male infertility. Materials and methods To search the related articles, Cochrane Library, EMBASE, ProQuest, Clinicaltrial.gov, WHO, Google Scholar, MEDLINE via Pubmed, Web of Science, SID, Magiran,...
Pavithra Chinnasamy1
Estimated H-index: 1
(Bharathiar University),
Rajendran Arumugam2
Estimated H-index: 2
(Bharathiar University)
Abstract The present study was designed to explore ethnopharmacological anti-inflammatory plants in the anti-cancer drug development. From the specialized local herbalists of the study area, who were treating tumors using anti-inflammatory plants by considering as a type of inflammation and explaining the potential of anti-inflammatory plants in prevalence of early stage's cancer. Interaction results obtained from the herbalists, and in silico PASS and CLC-pred prediction results were greatly ag...
Published on Feb 1, 2017in Saudi Journal of Biological Sciences 2.82
Sameer H. Qari2
Estimated H-index: 2
(UQU: Umm al-Qura University),
S.M. El-Assouli1
Estimated H-index: 1
(KAU: King Abdulaziz University)
Abstract The current study was carried out to evaluate the genotoxic aspects of the aqueous extracts of the Tribulus terrestris fruits by comet assay and cytogenetic procedures conditions on cultured human peripheral blood lymphocyte. After the treatment of the lymphocytes with four concentrations of the aqueous fruit extract of T. terrestris (10, 20, 40 and 80 mg/L) for 24 h it was noticed that, the presence of micronuclei and/or chromosomal aberration were monitored and a significant increase ...
Published on Jan 1, 2017
Andrzej Pokrywka10
Estimated H-index: 10
(University of Zielona Góra),
Barbara Morawin1
Estimated H-index: 1
(University of Zielona Góra)
+ 1 AuthorsAgnieszka Zembron-Lacny3
Estimated H-index: 3
(University of Zielona Góra)
Abstract Tribulus terrestris L. is an annual herbal plant of the family Zygophyllaceae, which was and is still used in different countries over the world for treatment of various kinds of diseases. It is used in traditional folk medicines as a tonic, aphrodisiac, palliative, astringent, stomachic, antihypertensive, diuretic, lithotriptic, and urinary disinfectant, as it is a component of numerous supplements that are available over the counter and widely recommended, generally as enhancers of hu...