Elevated uric acid correlates with wound severity.

Published on Apr 1, 2012in International Wound Journal 2.38
· DOI :10.1111/j.1742-481X.2011.00870.x
Melissa Laura Fernandez3
Estimated H-index: 3
(Queensland University of Technology),
Zee Upton29
Estimated H-index: 29
(Queensland University of Technology)
+ 2 AuthorsGary K. Shooter8
Estimated H-index: 8
(Queensland University of Technology)
Abstract
Chronic venous leg ulcers are a major health issue and represent an often overlooked area of biomedical research. Nevertheless, it is becoming increasingly evident that new approaches to enhance healing outcomes may arise through better understanding the processes involved in the formation of chronic wounds. We have for the first time shown that the terminal purine catabolite uric acid (UA) is elevated in wound fluid (WF) from chronic venous leg ulcers with relative concentrations correlating with wound chronicity. We have also shown a corresponding depletion in UA precursors, including adenosine, with increased wound severity. Further, we have shown that xanthine oxidase, the only enzyme in humans that catalyses the production of UA in conjunction with a burst of free radicals, is active in chronic WF. Taken together, this provides compelling evidence that xanthine oxidase may play a critical role in the formation of chronic wounds by prolonging the inflammatory process.
  • References (49)
  • Citations (24)
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References49
Published on Jan 1, 1996in Laboratory Investigation 4.26
A. Sarnesto1
Estimated H-index: 1
,
N. Linder1
Estimated H-index: 1
,
K. O. Raivio1
Estimated H-index: 1
Xanthine dehydrogenase/xanthine oxidase (XDH/XO) is a major cytoplasmic source of superoxide radicals and hydrogen peroxide, and it is considered important in the pathogenesis of ischemia-reperfusion damage. Because little is known about the enzyme in human tissues, the aims of this study were to purify human XDH/XO and to produce Ab for detection of the protein in Western blots and for quantification by ELISA. We purified human milk XDH/XO, produced Ab for Western blotting and ELISA of the prot...
92 Citations
Published on Jan 1, 2011in Sub-cellular biochemistry
Albert van der Vliet33
Estimated H-index: 33
(University of Vermont),
Peter F. Bove7
Estimated H-index: 7
(University of North Carolina at Chapel Hill)
Airway epithelia are continuously damaged by airborne pollutants, pathogens and allergens, and they rely on intrinsic mechanisms to restore barrier integrity. Epithelial repair is a multi-step process including cell migration into the wounded area, proliferation, differentiation and matrix deposition. Each step requires the secretion of various molecules, including growth factors, integrins and matrix metalloproteinases. Evidence is emerging that purinergic signaling promotes repair in human air...
11 Citations Source Cite
Published on Jan 1, 1998in Advances in Experimental Medicine and Biology 1.76
F. Gizzi1
Estimated H-index: 1
,
M. Papponetti1
Estimated H-index: 1
+ 4 AuthorsG. Spoto1
Estimated H-index: 1
Human chorionic gonadotrophin (hCG), alpha-feto protein (αFP), unconjugated 3-oestriol (uE3) are the markers currently used in prenatal serum screening programs for neural tube defects and chromosomal disorders (1). However the use of triple markers, when screening for foetal Down syndrome, still fails to detect 40% of Down syndrome in women less than 35 years of age. Other screening methods are currently under investigation (2). In Down syndrome, abnormalities of purine metabolism have been det...
4 Citations Source Cite
Published on Feb 1, 2010in Ostomy Wound Management 0.99
Hon J1
Estimated H-index: 1
,
Lagden K1
Estimated H-index: 1
+ 4 AuthorsWoodbury Mg1
Estimated H-index: 1
39 Citations
Published on Apr 1, 1990in Clinical pharmacy
Robert Parker1
Estimated H-index: 1
(Georgia Regents University),
P. L. McCollam1
Estimated H-index: 1
(Georgia Regents University)
This review discusses the pharmacology, pharmacokinetics, clinical efficacy, adverse effects, and dosage and administration of adenosine in the treatment of episodes of PSVT. ATP is also briefly reviewed, since the electrophysiologic effects of ATP in humans are believed to be due to its degradation to adenosine
27 Citations
Published on Jan 1, 1986in Acta physiologica Scandinavica
D. N. Granger56
Estimated H-index: 56
,
Höllwarth Me1
Estimated H-index: 1
,
Parks Da3
Estimated H-index: 3
477 Citations
Published on May 1, 1996in Clinical Chemistry 8.64
L Kuracka1
Estimated H-index: 1
,
Terézia Kalnovičová4
Estimated H-index: 4
+ 1 AuthorsPeter Turcani12
Estimated H-index: 12
We describe a convenient method for the separation and quantification of xanthine, hypoxanthine, and uric acid in 20 microL of cerebrospinal fluid (CSF) with use of HPLC and ultraviolet detection. The analysis is performed on a Sepharon SGX C18 column and the elution system consists of potassium phosphate buffer, pH 5.1, with 20 mL/L methanol. The lower limit of detection was 4 pmol for hypoxanthine and xanthine and 6 pmol for uric acid. Analytical recoveries of purine metabolites ranged from 98...
21 Citations
Published on Mar 1, 1961in Annals of Internal Medicine 19.38
Daniel J. McCarty49
Estimated H-index: 49
,
Joseph Lee Hollander9
Estimated H-index: 9
325 Citations Source Cite
Published on Dec 7, 2007in British Journal of Dermatology 6.13
Melissa Laura Fernandez3
Estimated H-index: 3
(Queensland University of Technology),
James A. Broadbent6
Estimated H-index: 6
(Queensland University of Technology)
+ 2 AuthorsZee Upton29
Estimated H-index: 29
(Queensland University of Technology)
Summary Background Chronic venous leg ulcers are a significant cause of pain, immobility and decreased quality of life for patients with these wounds. In view of this, research efforts are focused on multiple factors in the wound environment to obtain information regarding the healing of ulcers. Objectives Chronic wound fluid (CWF), containing a complex mixture of proteins, is an important modulator of the wound environment, and therefore we hypothesized that these proteins may be indicators of ...
34 Citations Source Cite
Published on May 1, 1993in The EMBO Journal 10.56
M. Meyer1
Estimated H-index: 1
(Ludwig Maximilian University of Munich),
R. Schreck1
Estimated H-index: 1
(Ludwig Maximilian University of Munich),
Patrick A. Baeuerle9
Estimated H-index: 9
(Ludwig Maximilian University of Munich)
We show that AP-1 is an antioxidant-responsive transcription factor. DNA binding and transactivation by AP-1 were induced in HeLa cells upon treatment with the antioxidants pyrrolidine dithiocarbamate (PDTC) and N-acetyl-L-cysteine (NAC), and upon transient expression of the antioxidative enzyme thioredoxin. While PDTC and NAC enhanced DNA binding and transactivation of AP-1 in response to phorbol ester, the oxidant H2O2 suppressed phorbol ester activation of the factor. H2O2 on its own was only...
1,174 Citations Source Cite
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  • Citations (24)
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Cited By24
Published on Jun 1, 2015in Applied Microbiology and Biotechnology 3.34
Gregor Tegl5
Estimated H-index: 5
(University of Natural Resources and Life Sciences, Vienna),
Doris Schiffer4
Estimated H-index: 4
(University of Natural Resources and Life Sciences, Vienna)
+ 2 AuthorsGeorg Gübitz57
Estimated H-index: 57
(University of Natural Resources and Life Sciences, Vienna)
Wound infection is a severe complication causing delayed healing and risks for patients. Conventional methods of diagnosis for infection involve error-prone clinical description of the wound and time-consuming microbiological tests. More reliable alternatives are still rare, except for invasive and unaffordable gold standard methods. This review discusses the diversity of new approaches for wound infection determination. There has been progress in the detection methods of microorganisms, includi...
12 Citations Source Cite
Published on Feb 1, 2014in Current Rheumatology Reports 3.08
Melissa Laura Fernandez3
Estimated H-index: 3
(Queensland University of Technology),
Zee Upton29
Estimated H-index: 29
(Queensland University of Technology),
Gary K. Shooter8
Estimated H-index: 8
(Queensland University of Technology)
Chronic wounds are an important health problem because they are difficult to heal and treatment is often complicated, lengthy and expensive. For a majority of sufferers the most common outcomes are long-term immobility, infection and prolonged hospitalisation. There is therefore an urgent need for effective therapeutics that will enhance ulcer healing and patient quality of life, and will reduce healthcare costs. Studies in our laboratory have revealed elevated levels of purine catabolites in wo...
7 Citations Source Cite
Published on Mar 1, 2013in Biosensors and Bioelectronics 8.17
Tim R. Dargaville20
Estimated H-index: 20
(Queensland University of Technology),
Brooke L. Farrugia12
Estimated H-index: 12
(Queensland University of Technology)
+ 3 AuthorsH VoelckerNicolas44
Estimated H-index: 44
(University of South Australia)
Abstract Wound healing involves a complex series of biochemical events and has traditionally been managed with ‘low tech’ dressings and bandages. The concept that diagnostic and theranostic sensors can complement wound management is rapidly growing in popularity as there is tremendous potential to apply this technology to both acute and chronic wounds. Benefits in sensing the wound environment include reduction of hospitalization time, prevention of amputations and better understanding of the pr...
154 Citations Source Cite
Published on Mar 1, 2014in Electrochemistry Communications 4.66
Anna McLister4
Estimated H-index: 4
(Ulster University),
Jolene Phair6
Estimated H-index: 6
(Ulster University)
+ 1 AuthorsJames Davis29
Estimated H-index: 29
(Ulster University)
Abstract At present, there is a clear requirement for the development of new sensors or the translation of existing systems for the point of care management of chronic wounds. The availability of such devices could significantly improve clinical outcome — particularly in the early identification of infection. This review identifies electrochemical research strategies that are being targeted at wound diagnostics and provides a critical overview of emerging research that could be pivotal in settin...
12 Citations Source Cite
Published on Mar 1, 2014in Sensors and Actuators B-chemical 5.67
Jolene Phair6
Estimated H-index: 6
(Ulster University),
John Benson3
Estimated H-index: 3
(Ulster University)
+ 5 AuthorsJames Davis29
Estimated H-index: 29
(Ulster University)
Butyl grafted polyethylene film loaded with carbon was investigated as a mechanically flexible conductive substrate for electrochemical sensing applications. The electroanalytical properties of the film before and after laser modification and electrochemical anodisation were assessed and its potential application for use in monitoring wound healing critically evaluated using uric acid as a biomarker. The design and development of a prototype smart bandage is detailed and the response to urate ha...
4 Citations Source Cite
Published on Aug 1, 2013in Electrochemistry Communications 4.66
Jolene Phair6
Estimated H-index: 6
(Ulster University),
James Davis29
Estimated H-index: 29
(Ulster University)
+ 1 AuthorsMarco F. Cardosi3
Estimated H-index: 3
Atmospheric pressure plasma processing has been investigated as a rapid means of activating carbon fibre thread for use in the manufacture of smart bandages. Ferrocyanide and ruthenium hexamine were used as redox probes to assess the influence of plasma exposure time on the electroanalytical performance of material to be incorporated within prototype wound dressings. Ion exchange of the ruthenium at the carbon interface was observed and was attributed to the plasma induced creation of surface ca...
8 Citations Source Cite
Published on Feb 1, 2014in Materials Chemistry and Physics 2.21
Jolene Phair6
Estimated H-index: 6
(Ulster University),
Mayank Joshi1
Estimated H-index: 1
(Ulster University)
+ 2 AuthorsJames Davis29
Estimated H-index: 29
(Ulster University)
Carbon loaded polyethylene films were selected as the base substrate for a mechanically flexible and conductive sensing material for use wound monitoring technologies. The films were processed using laser ablation of the surface to increase the effective surface area of the electrode and then subject to an oxidative electrochemical etch to improve the electron transfer kinetics. The surface morphology of the resulting films was analysed and the electrode performance in relation to monitoring uri...
9 Citations Source Cite
Published on Apr 5, 2014in Chemistry Letters 1.63
Jolene Phair6
Estimated H-index: 6
,
Andy Brown16
Estimated H-index: 16
+ 6 AuthorsJames Davis29
Estimated H-index: 29
Carbon–polycarbonate films were investigated as a conductive substrate with the potential for monitoring chronic wounds. The films were found to be mechanically flexible and could be easily integrated within conventional dressing materials. The ability of the films to selectively monitor urate (as an endogenous pH probe) were investigated. A preliminary assessment of its performance in simulated wound fluid was conducted
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Published on Jul 1, 2015in Electrochemistry Communications 4.66
Petar Kassal1
Estimated H-index: 1
(University of California, San Diego),
Jayoung Kim12
Estimated H-index: 12
(University of California, San Diego)
+ 4 AuthorsJoseph Wang122
Estimated H-index: 122
(University of California, San Diego)
Abstract Advanced wound care technologies need to evolve in response to the growing burden of chronic wounds on national healthcare budgets and the debilitating impact chronic wounds have on patient quality of life. We describe here a new type of smart bandage for determination of uric acid (UA) status, a key wound biomarker, formed by screen printing an amperometric biosensor directly on a wound dressing. Immobilized uricase, paired with a printed catalytic Prussian blue transducer, facilitates...
47 Citations Source Cite
Published on May 1, 2014in Journal of Pain and Symptom Management 3.25
Helen Edwards36
Estimated H-index: 36
(Queensland University of Technology),
Kathleen Finlayson19
Estimated H-index: 19
(Queensland University of Technology)
+ 4 AuthorsMichelle Gibb7
Estimated H-index: 7
(Queensland University of Technology)
Abstract Context Patients with venous leg ulcers experience multiple symptoms, including pain, depression, and discomfort from lower leg inflammation and wound exudate. Some of these symptoms impair wound healing and decrease quality of life (QOL). The presence of co-occurring symptoms may have a negative effect on these outcomes. The identification of symptom clusters could potentially lead to improvements in symptom management and QOL. Objectives To identify the prevalence and severity of comm...
19 Citations Source Cite