Branding/Logomark minus Citation Combined Shape Icon/Bookmark-empty Icon/Copy Icon/Collection Icon/Close Copy 7 no author result Created with Sketch. Icon/Back Created with Sketch. Match!

A role for iron and oxygen chemistry in preserving soft tissues, cells and molecules from deep time

Published on Nov 27, 2013
· DOI :10.1098/rspb.2013.2741
Mary H. Schweitzer24
Estimated H-index: 24
(NCSU: North Carolina State University),
Wenxia Zheng10
Estimated H-index: 10
(NCSU: North Carolina State University)
+ 5 AuthorsSirine C. Fakra35
Estimated H-index: 35
(LBNL: Lawrence Berkeley National Laboratory)
Abstract
The persistence of original soft tissues in Mesozoic fossil bone is not explained by current chemical degradation models. We identified iron particles (goethite-αFeO(OH)) associated with soft tissues recovered from two Mesozoic dinosaurs, using transmission electron microscopy, electron energy loss spectroscopy, micro-X-ray diffraction and Fe micro-X-ray absorption near-edge structure. Iron chelators increased fossil tissue immunoreactivity to multiple antibodies dramatically, suggesting a role for iron in both preserving and masking proteins in fossil tissues. Haemoglobin (HB) increased tissue stability more than 200-fold, from approximately 3 days to more than two years at room temperature (25°C) in an ostrich blood vessel model developed to test post-mortem ‘tissue fixation’ by cross-linking or peroxidation. HB-induced solution hypoxia coupled with iron chelation enhances preservation as follows: HB + O2 > HB − O2 > −O2 ≫ +O2. The well-known O2/haeme interactions in the chemistry of life, such as respiration and bioenergetics, are complemented by O2/haeme interactions in the preservation of fossil soft tissues.
Figures & Tables
  • References (88)
  • Citations (35)
Cite
References88
Newest
Dale E. Greenwalt5
Estimated H-index: 5
,
Yulia Goreva2
Estimated H-index: 2
(National Museum of Natural History)
+ 2 AuthorsRalph E. Harbach46
Estimated H-index: 46
(Natural History Museum)
Abstract Although hematophagy is found in ∼14,000 species of extant insects, the fossil record of blood-feeding insects is extremely poor and largely confined to specimens identified as hematophagic based on their taxonomic affinities with extant hematophagic insects; direct evidence of hematophagy is limited to four insect fossils in which trypanosomes and the malarial protozoan Plasmodium have been found. Here, we describe a blood-engorged mosquito from the Middle Eocene Kishenehn Formation in...
45 Citations Source Cite
Published on Jul 1, 2013in Nature 41.58
Ludovic Orlando52
Estimated H-index: 52
(UCPH: University of Copenhagen),
Aurélien Ginolhac17
Estimated H-index: 17
(UCPH: University of Copenhagen)
+ 53 AuthorsIda Moltke25
Estimated H-index: 25
(U of C: University of Chicago)
A low-coverage draft genome sequence from a horse bone recovered from permafrost dated to approximately 560–780 thousand years ago is presented; this represents the oldest full genome sequence to date by almost an order of magnitude.
349 Citations Source Cite
Published on Mar 1, 2013in Journal of Biological Inorganic Chemistry 2.95
Carolyn Dehner6
Estimated H-index: 6
(ND: University of Notre Dame),
Nydia Morales-Soto8
Estimated H-index: 8
(ND: University of Notre Dame)
+ 4 AuthorsJennifer L. DuBois16
Estimated H-index: 16
(SRI: SRI International)
Metabolism of iron derived from insoluble and/or scarce sources is essential for pathogenic and environmental microbes. The ability of Pseudomonas aeruginosa to acquire iron from exogenous ferritin was assessed; ferritin is an iron-concentrating and antioxidant protein complex composed of a catalytic protein and caged ferrihydrite nanomineral synthesized from Fe(II) and O2 or H2O2. Ferritin and free ferrihydrite supported growth of P. aeruginosa with indistinguishable kinetics and final culture ...
19 Citations Source Cite
Published on Jan 1, 2013in Coordination Chemistry Reviews 14.50
Elizabeth C. Theil49
Estimated H-index: 49
(Children's Hospital Oakland Research Institute),
Rabindra Kumar Behera10
Estimated H-index: 10
(Children's Hospital Oakland Research Institute),
Takehiko Tosha2
Estimated H-index: 2
(Children's Hospital Oakland Research Institute)
Abstract Ferritins, highly symmetrical protein nanocages, are reactors for Fe 2+ and dioxygen or hydrogen peroxide that are found in all kingdoms of life and in many different cells of multicellular organisms. They synthesize iron concentrates required for cells to make cofactors of iron proteins (heme, FeS, mono and diiron). The caged ferritin biominerals, Fe 2 O 3 ·H 2 O are also antioxidants, acting as sinks for iron and oxidants scavenged from damaged proteins; genetic regulation of ferritin...
79 Citations Source Cite
Published on Jan 1, 2013in Bone 4.46
Mary H. Schweitzer24
Estimated H-index: 24
(NCSU: North Carolina State University),
Wenxia Zheng10
Estimated H-index: 10
(NCSU: North Carolina State University)
+ 1 AuthorsMarshall W. Bern40
Estimated H-index: 40
(PARC)
article i nfo The discovery of soft, transparent microstructures in dinosaur bone consistent in morphology with osteocytes was controversial. We hypothesize that, if original, these microstructures will have molecular features in com- mon with extant osteocytes. We present immunological and mass spectrometry evidence for preservation of proteins comprising extant osteocytes (Actin, Tubulin, PHEX, Histone H4) in osteocytes recovered from two non-avian dinosaurs. Furthermore, antibodies to DNA sho...
38 Citations Source Cite
Published on Dec 7, 2012
Morten E. Allentoft25
Estimated H-index: 25
(UCPH: University of Copenhagen),
Matthew J. Collins51
Estimated H-index: 51
(Ebor: University of York)
+ 11 AuthorsEske Willerslev90
Estimated H-index: 90
(UCPH: University of Copenhagen)
Claims of extreme survival of DNA have emphasized the need for reliable models of DNA degradation through time. By analysing mitochondrial DNA (mtDNA) from 158 radiocarbon-dated bones of the extinct New Zealand moa, we confirm empirically a long-hypothesized exponential decay relationship. The average DNA half-life within this geographically constrained fossil assemblage was estimated to be 521 years for a 242 bp mtDNA sequence, corresponding to a per nucleotide fragmentation rate (k) of 5.50 × ...
213 Citations Source Cite
Published on Sep 1, 2012in Bone 4.46
Edwin Alberto Cadena Rueda11
Estimated H-index: 11
(NCSU: North Carolina State University),
Mary H. Schweitzer24
Estimated H-index: 24
(NCSU: North Carolina State University)
article i nfo Here we describe variations in osteocytes derived from each of the three bone layers that comprise the turtle shell. We examine osteocytes in bone from four extant turtle species to form a morphological 'baseline' ,a nd then compare these with morphologies of osteocytes preserved in Cenozoic and Mesozoic fossils. Two different morphotypes of osteocytes are recognized: flattened-oblate osteocytes (FO osteocytes), which are particularly abundant in the internal cortex and lamellae of...
18 Citations Source Cite
Published on May 1, 2012in Forensic Science International-genetics 5.64
Reza Alaeddini3
Estimated H-index: 3
(American Board of Legal Medicine)
Abstract Polymerase chain reaction (PCR) is currently the method of choice for the identification of human remains in forensic coursework. DNA samples from crime scenes often contain co-purified impurities which inhibit PCR. PCR inhibition is the most common cause of PCR failure when adequate copies of DNA are present. Inhibitors have been routinely reported in forensic investigations of DNA extracted from a variety of templates. Humic compounds, a series of substances produced during decay proc...
131 Citations Source Cite
Published on Oct 1, 2011in Biochimica et Biophysica Acta 3.44
Andrea Bellelli27
Estimated H-index: 27
(Sapienza University of Rome),
Maurizio Brunori61
Estimated H-index: 61
(Sapienza University of Rome)
Abstract The two-state allosteric model of Monod, Wyman and Changeux (1965) offers a simple and elegant, yet very powerful and comprehensive, description of the functional behavior of hemoglobin. Although the extensive body of structural and functional information available is by-and-large consistent with this conceptual framework, some discrepancies between theory and experiment have been extensively discussed and considered to demand modifications of the original hypothesis. More recently the ...
25 Citations Source Cite
Published on May 30, 2011in Annual Review of Earth and Planetary Sciences 11.39
Mary H. Schweitzer24
Estimated H-index: 24
(NCSU: North Carolina State University)
Exceptionally preserved fossils—i.e., those that retain, in some manner, labile components of organisms that are normally degraded far too quickly to enter the fossil record—hold the greatest potential for understanding aspects of the biology of long-extinct animals and are the best targets for the search for endogenous biomolecules. Yet the modes of preservation of these labile components, and exactly what remains of the original composition, are not well understood. Here, I review a selection ...
53 Citations Source Cite
Cited By35
Newest
Published on Apr 23, 2019in Scientific Reports 4.12
Victoria E. McCoy7
Estimated H-index: 7
(University of Bonn),
Sarah E. Gabbott21
Estimated H-index: 21
(University of Leicester)
+ 8 AuthorsXavier Delclòs18
Estimated H-index: 18
(University of Barcelona)
Ancient protein analysis is a rapidly developing field of research. Proteins ranging in age from the Quaternary to Jurassic are being used to answer questions about phylogeny, evolution, and extinction. However, these analyses are sometimes contentious, and focus primarily on large vertebrates in sedimentary fossilisation environments; there are few studies of protein preservation in fossils in amber. Here we show exceptionally slow racemisation rates during thermal degradation experiments of re...
Source Cite
Published on Jul 1, 2019in Palaeogeography, Palaeoclimatology, Palaeoecology 2.38
Daria Kiseleva , Oleg Shilovsky (Kazan: Kazan Federal University)+ 4 AuthorsNadezhda Cherednichenko
Abstract The Kotelnich vertebrate fossil site, one of the richest of the Permian period, is characterised by the excellent preservation of fossil remains due to their burial in a silty anaerobic environment similar to modern peat bogs. The aim of the work is to carry out a comprehensive study of the composition and structure of bone fragments of the Permian parareptile Deltavjatia vjatkensis , as well as that of its embedding rocks, to support further paleoecological and fossilisation reconstruc...
Source Cite
Published on Jul 1, 2019in Cretaceous Research 1.93
Paul V. Ullmann2
Estimated H-index: 2
(Rowan University),
Suraj H. Pandya (Drexel University), Ron Nellermoe1
Estimated H-index: 1
(Concordia College)
Abstract Recovery of soft tissues and cells from fossil bones is becoming increasingly common, with structures morphologically consistent with vertebrate osteocytes, blood vessels, fibrous/collagenous matrix, and potential intravascular contents now recognized from specimens dating back to the Permian. However, it largely remains unclear how bone tissue structure, early diagenetic regimes, and many other taphonomic variables influence or control the preservation potential of soft tissues in vert...
Source Cite
Published on Jan 1, 2019in eLife 7.62
Evan T. Saitta1
Estimated H-index: 1
,
R Liang + 16 AuthorsGeoffrey D. Abbott22
Estimated H-index: 22
Maicon Sebold1
Estimated H-index: 1
(State University of Campinas),
Carolina Bosso André7
Estimated H-index: 7
(State University of Campinas)
+ 1 AuthorsMarcelo Giannini36
Estimated H-index: 36
(State University of Campinas)
Abstract Objectives To evaluate the effect of experimental conditioners (10-3 solution − 10-3, 6.8% ferric oxalate - FOX, and 1.4% nitric acid - NI) on dentin elastic modulus, flexural strength, bond strength, failure mode, and adhesive interface morphology of two etch-and-rinse adhesives (XP Bond, Dentsply or One-Step, Bisco) applied on etched dry dentin. Methods Sound human third molars were used for the microtensile bond strength test (n=8), performed at 24 hours and after one year of water s...
Source Cite
Published on Apr 9, 2019in Historical Biology 1.25
Dawid Surmik3
Estimated H-index: 3
(University of Silesia in Katowice),
Mateusz Dulski12
Estimated H-index: 12
(University of Silesia in Katowice)
+ 2 AuthorsR. Pawlicki (Jagiellonian University Medical College)
Source Cite
Published on Dec 1, 2018
Anezka Popovski Kolaceke1
Estimated H-index: 1
(University of Regina),
Ryan C. McKellar12
Estimated H-index: 12
(KU: University of Kansas),
Mauricio Barbi1
Estimated H-index: 1
(University of Regina)
Synchrotron-based techniques offer a wealth of elemental, molecular, and structural insights in biological samples, but the application of these techniques to fossils is a relatively new development. Here we examine how synchrotron radiation micro X-ray fluorescence (SR µXRF) may be used to investigate the chemical composition of insects trapped in amber, while leaving the inclusions unaltered. Elemental distribution data could provide important information on tissue preservation in insect inclu...
1 Citations Source Cite
Published on Dec 1, 2018in Nature Communications 12.35
Jasmina Wiemann3
Estimated H-index: 3
(Yale University),
Matteo Fabbri5
Estimated H-index: 5
(Yale University)
+ 4 AuthorsDerek E. G. Briggs62
Estimated H-index: 62
(Yale University)
Vertebrate hard tissues consist of mineral crystallites within a proteinaceous scaffold that normally degrades post-mortem. Here we show, however, that decalcification of Mesozoic hard tissues preserved in oxidative settings releases brownish stained extracellular matrix, cells, blood vessels, and nerve projections. Raman Microspectroscopy shows that these fossil soft tissues are a product of diagenetic transformation to Advanced Glycoxidation and Lipoxidation End Products, a class of N-heterocy...
5 Citations Source Cite
Published on Nov 28, 2018in PLOS ONE 2.77
Mary Higby Schweitzer3
Estimated H-index: 3
(NCSU: North Carolina State University),
Wenxia Zheng10
Estimated H-index: 10
(NCSU: North Carolina State University)
+ 2 AuthorsJohan Lindgren20
Estimated H-index: 20
1 Citations Source Cite