Match!

hOGG1-Cys326 variant cells are hypersensitive to DNA repair inhibition by nitric oxide

Published on Jun 1, 2014in Carcinogenesis4.00
· DOI :10.1093/carcin/bgu066
Eva Moritz4
Estimated H-index: 4
(University of Mainz),
Karin Pauly5
Estimated H-index: 5
+ 3 AuthorsBernd Epe43
Estimated H-index: 43
Cite
Abstract
The repair of 8-oxo-7,8-dihydroguanine in the DNA of mammalian cells is initiated by 8-oxoguanine DNA glycosylase (OGG1). A frequent polymorphism in the human OGG1 gene, rs1052133, causes the substitution of serine by cysteine at amino acid 326 of the protein and has been associated with an altered risk for various types of cancer in some populations. The OGG1-Cys326 protein appears to have normal enzymatic activity, but greater sensitivity to oxidation than the serine variant. Here, we describe a comparison of the cellular repair by the two OGG1 variants under stress conditions characteristic of inflammation, namely in cells pretreated with nitric oxide (NO) or pre-exposed to hyperthermia. The results show that NO at concentrations causing negligible DNA damage and little cytotoxicity strongly reduces the repair rates of oxidized purines in the DNA of HeLa cells overexpressing the OGG1-Cys326 variant. The reduction in repair was much less pronounced in isogenic cells overexpressing the OGG1-Ser326 variant. Similar results were observed in EBVtransformed lymphocytes from donors homozygous for the two OGG1 variant alleles. In contrast, hyperthermia-induced stress caused a repair retardation that was independent of the OGG1 polymorphism. The repair inhibition by NO in the variant cells gave rise to increased genetic instability, measured as increased micronuclei formation after oxidant exposure. The results could explain a higher risk of malignant transformation in inflamed tissues of carriers of this variant allele.
  • References (67)
  • Citations (9)
Cite
References67
Newest
Published on Feb 1, 2014in Biochimica et Biophysica Acta3.79
Henrik E. Poulsen17
Estimated H-index: 17
(Copenhagen University Hospital),
Laia Llovera Nadal1
Estimated H-index: 1
(UCPH: University of Copenhagen)
+ 2 AuthorsAllan Weimann25
Estimated H-index: 25
(Copenhagen University Hospital)
Abstract Background DNA and RNA oxidations have been linked to diseases such as cancer, arteriosclerosis, neurodegeneration and diabetes. The prototype base modification studied is the 8-hydroxylation of guanine. DNA integrity is maintained by elaborate repair systems and RNA integrity is less studied but relies mainly on degradation. Scope of review DNA and RNA oxidations are measured by very similar techniques. The scope of this review is to highlight the preferred methods of measurement of ox...
Published on Oct 1, 2013in Free Radical Biology and Medicine5.66
Valeria Simonelli7
Estimated H-index: 7
(ISS: Istituto Superiore di Sanità),
Serena Camerini14
Estimated H-index: 14
(ISS: Istituto Superiore di Sanità)
+ 14 AuthorsSimonetta Guarrera32
Estimated H-index: 32
8-Oxoguanine DNA glycosylase (OGG) activity was measured by an in vitro assay in lymphocytes of healthy volunteers genotyped for various OGG1 polymorphisms. Only homozygous carriers of the polymorphic C326 allele showed a significantly lower OGG activity compared to the homozygous S326 genotype. The purified S326C OGG1 showed a decreased ability to complete the repair synthesis step in a base excision repair reaction reconstituted in vitro. The propensity of this variant to dimerize as well as i...
Frédérique Berger8
Estimated H-index: 8
(Curie Institute),
Laurence Vaslin6
Estimated H-index: 6
(Curie Institute)
+ 5 AuthorsJanet Hall40
Estimated H-index: 40
(Curie Institute)
Abstract The age at onset of Huntington disease (HD) shows a strong, negative correlation with the number of CAG repeats within the huntingtin ( HTT ) gene. However, this does not account for all the inter-individual variability seen among patients. In order to assess whether single-nucleotide polymorphisms (SNPs) in the OGG1 and XPC genes, both implicated in responses to oxidative stress, are associated with the age of onset of HD, 9 SNPs have been genotyped in 299 individuals with HD and 582 c...
Published on Apr 1, 2013in Toxicology Letters3.50
Guixiang Ji18
Estimated H-index: 18
,
Lifeng Yan8
Estimated H-index: 8
(Nanjing Medical University)
+ 2 AuthorsAihua Gu20
Estimated H-index: 20
(Nanjing Medical University)
Abstract 8-Oxoguanine DNA glycosylase 1 (OGG1) plays an important role in repairing oxidative DNA damage induced by chemical agents, such as tobacco. This study examined the effects of OGG1 Ser326Cys polymorphism and cigarette smoking, alone or combined, on sperm oxidative DNA damage and the risk of male infertility. A total of 620 idiopathic infertile subjects and 480 fertile controls were recruited in this study. Sperm 8-hydroxydeoxyguanine (8-OHdG) was measured by immunofluorescent assay usin...
Published on Mar 1, 2013in DNA Repair3.71
Damiano Fantini6
Estimated H-index: 6
,
Eva Moritz4
Estimated H-index: 4
(University of Mainz)
+ 5 AuthorsJ. Pablo Radicella28
Estimated H-index: 28
Abstract Inhibition of DNA repair has been proposed as a mechanism underlying heat-induced sensitization of tumour cells to some anticancer treatments. Base excision repair (BER) constitutes the main pathway for the repair of DNA lesions induced by oxidizing or alkylating agents. Here, we report that mild hyperthermia, without toxic consequences per se, affects cellular DNA glycosylase activities, thus impairing BER. Exposure of cells to mild hyperthermia leads to a rapid and selective inactivat...
Published on Dec 1, 2012in Molecular Biology Reports2.11
Wu Wei7
Estimated H-index: 7
(Changzhi Medical College),
Xiao-Feng He10
Estimated H-index: 10
(Changzhi Medical College)
+ 5 AuthorsWei Wang3
Estimated H-index: 3
(University of Fukui)
The previous published data on the association between the 8-oxo-guanine glycosylase-1 (OGG1) and apurinic/apyrimidinic-endonuclease-1 (APEX1/APE1) polymorphisms and lung cancer risk remained controversial. Several polymorphisms in the OGG1 and APEX1 gene have been described, including the commonly occurring Ser326Cys in OGG1 and Asp148Glu in APEX1. This meta-analysis of literatures was performed to derive a more precise estimation of the relationship. A total of 37 studies were identified to th...
Published on Oct 1, 2012in Human & Experimental Toxicology2.17
Bensu Karahalil23
Estimated H-index: 23
(Gazi University),
Vilhelm A. Bohr87
Estimated H-index: 87
(NIH: National Institutes of Health),
Dm Wilson1
Estimated H-index: 1
(NIH: National Institutes of Health)
Genetic variation in DNA repair genes can modulate DNA repair capacity and may be related to cancer risk. However, study findings have been inconsistent. Inheritance of variant DNA repair genes is believed to influence individual susceptibility to the development of environmental cancer. Reliable knowledge on which the base excision repair (BER) sequence variants are associated with cancer risk would help elucidate the mechanism of cancer. Given that most of the previous studies had inadequate s...
Published on Sep 13, 2012in Viruses3.81
Lori Frappier42
Estimated H-index: 42
(U of T: University of Toronto)
Epstein–Barr virus (EBV) immortalizes host cells as part of its latent mode of infection. As a result of this ability to promote cell proliferation and survival, EBV infection contributes to the development of several kinds of B-cell lymphomas and epithelial tumours. The EBV Epstein–Barr nuclear antigen 1 (EBNA1) protein is the only EBV protein expressed in all EBV-associated tumours and plays multiple important roles in EBV latency. In addition to its well-studied roles in viral DNA replication...
Published on Aug 1, 2012in Mutation Research
Fulvio Ricceri40
Estimated H-index: 40
(UNITO: University of Turin),
Giuseppe Matullo49
Estimated H-index: 49
(UNITO: University of Turin),
Paolo Vineis110
Estimated H-index: 110
Abstract DNA suffers from a wide range of damage, both from extracellular agents and via endogenous mechanisms. Damage of DNA can lead to cancer and other diseases. Therefore, it is plausible that sequence variants in DNA repair genes are involved in cancer development. A recent systematic review and meta-analysis, based on the “Venice criteria”, showed that out of 241 associations investigated, only three resulted to have a strong grade of cumulative evidence. These associations were: two SNPs ...
Published on Jul 1, 2012in Mutagenesis2.90
Rachael M. Kershaw4
Estimated H-index: 4
(University of Birmingham),
Nikolas J. Hodges16
Estimated H-index: 16
(University of Birmingham)
Gene–environment interactions influence an individual’s risk of disease development. A common human 8oxoguanine DNA glycosylase 1 (OGG1) variant, Cys326hOGG1, has been associated with increased cancer risk. Evidence suggests that this is due to reduced repair ability, particularly under oxidising conditions but the underlying mechanism is poorly understood. Oxidising conditions may arise due to internal cellular processes, such as inflammation or external chemical or radiation exposure. To inves...
Cited By9
Newest
Published on May 1, 2019in Journal of Cellular Physiology4.52
Bagher Farhood10
Estimated H-index: 10
(KAUMS: Kashan University of Medical Sciences),
Nasser Hashemi Goradel4
Estimated H-index: 4
(Tehran University of Medical Sciences)
+ 3 AuthorsAmirhossein Sahebkar53
Estimated H-index: 53
(MUMS: Mashhad University of Medical Sciences)
Published on Aug 1, 2018in Reproductive Toxicology3.20
Samantha M. Anderson1
Estimated H-index: 1
(UKZN: University of KwaZulu-Natal),
Rajen N. Naidoo16
Estimated H-index: 16
(UKZN: University of KwaZulu-Natal)
+ 3 AuthorsAnil A. Chuturgoon20
Estimated H-index: 20
(UKZN: University of KwaZulu-Natal)
Abstract The global HIV and obesity epidemics are major public health concerns; particularly as both are associated with increased risk of adverse birth outcomes. Despite extensive research, their combined effect, in terms of birth outcomes, has not been investigated. A single-nucleotide polymorphism (SNP) within 8-oxoguanine glycosylase 1 (OGG1) (Ser326Cys) has been suggested to affect body mass indices and therefore could predispose South African (SA) women to adverse effects of obesity. This ...
Published on Aug 19, 2017in International Journal of Molecular Sciences4.18
Shosukekawanishi62
Estimated H-index: 62
,
Shiho Ohnishi13
Estimated H-index: 13
+ 2 AuthorsMariko Murata38
Estimated H-index: 38
Inflammation can be induced by chronic infection, inflammatory diseases and physicochemical factors. Chronic inflammation is estimated to contribute to approximately 25% of human cancers. Under inflammatory conditions, inflammatory and epithelial cells release reactive oxygen (ROS) and nitrogen species (RNS), which are capable of causing DNA damage, including the formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine and 8-nitroguanine. We reported that 8-nitroguanine was clearly formed at the sites o...
Published on Jun 1, 2017in Free Radical Biology and Medicine5.66
Marco Seifermann3
Estimated H-index: 3
(University of Mainz),
Bernd Epe43
Estimated H-index: 43
(University of Mainz)
Abstract The generation of DNA modifications in cells is in most cases accidental and associated with detrimental consequences such as increased mutation rates and an elevated risk of malignant transformation. Accordingly, repair enzymes involved in the removal of the modifications have primarily a protective function. Among the well-established exceptions of this rule are 5-methylcytosine and uracil, which are generated in DNA enzymatically under controlled conditions and fulfill important regu...
Published on Jun 1, 2017in Free Radical Biology and Medicine5.66
Mariarosaria D’Errico8
Estimated H-index: 8
(ISS: Istituto Superiore di Sanità),
Eleonora Parlanti14
Estimated H-index: 14
(ISS: Istituto Superiore di Sanità)
+ 4 AuthorsEugenia Dogliotti40
Estimated H-index: 40
(ISS: Istituto Superiore di Sanità)
Abstract Oxidative stress is associated with a growing number of diseases that span from cancer to neurodegeneration. Most oxidatively induced DNA base lesions are repaired by the base excision repair (BER) pathway which involves the action of various DNA glycosylases. There are numerous genome wide studies attempting to associate single-nucleotide polymorphisms (SNPs) with predispositions to various types of disease; often, these common variants do not have significant alterations in their bioc...
Published on May 3, 2016in Oncotarget
Carlos Benitez-Buelga4
Estimated H-index: 4
,
Tereza Vaclova3
Estimated H-index: 3
+ 6 AuthorsJavier Benitez68
Estimated H-index: 68
// Carlos Benitez-Buelga 1 , Tereza Vaclova 1 , Sofia Ferreira 1 , Miguel Urioste 2, 5 , Lucia Inglada-Perez 3, 5 , Nora Soberon 4 , Maria A. Blasco 4 , Ana Osorio 1, 5 , Javier Benitez 1, 5 1 Human Genetics Group, Spanish National Cancer Research Center (CNIO), Madrid 28029, Spain 2 Familial Cancer Clinical Unit, Spanish National Cancer Research Center (CNIO), Madrid 28029, Spain 3 Endocrine Cancer Group, Spanish National Cancer Research Center (CNIO), Madrid 28029, Spain 4 Telomere and Telomer...
Published on Sep 1, 2015in Journal of Affective Disorders4.08
Piotr Czarny9
Estimated H-index: 9
(University of Łódź),
Dominik Kwiatkowski7
Estimated H-index: 7
(University of Łódź)
+ 8 AuthorsKuan-Pin Su27
Estimated H-index: 27
(PRC: China Medical University (PRC))
Abstract Background An elevated levels oxidative modified DNA bases and a decreased efficiency of oxidative DNA damage repair were found in patients with depression disorders, including recurrent type (rDD). The glycosylases are involved in base excision repair (BER), which eliminates oxidative DNA damage. Therefore, we genotyped the single nucleotide polymorphisms (SNPs) of genes encoding three glycosylases: hOGG1, MUTYH and NEIL1. Methods We selected three polymorphisms: c.977C>G – hOGG1 (rs10...
Published on Feb 1, 2015in DNA Repair3.71
Jordan Morreall3
Estimated H-index: 3
(Emory University),
Kristin L. Limpose3
Estimated H-index: 3
(Emory University)
+ 3 AuthorsPaul W. Doetsch38
Estimated H-index: 38
Abstract Reactive oxygen species threaten genomic integrity by inducing oxidative DNA damage. One common form of oxidative DNA damage is the mutagenic lesion 8-oxoguanine (8-oxodG). One driver of oxidative stress that can induce 8-oxodG is inflammation, which can be initiated by the cytokine tumor necrosis factor alpha (TNF-α). Oxidative DNA damage is primarily repaired by the base excision repair pathway, initiated by glycosylases targeting specific DNA lesions. 8-oxodG is excised by 8-oxoguani...