Re‐face stereospecificity of NADP dependent methylenetetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 as determined by NMR spectroscopy

MtdA catalyzes the dehydrogenation of N 5 , N 10 ‐methylenetetrahydromethanopterin (methylene‐H 4 MPT) with NADP + as electron acceptor. In the reaction two prochiral centers are involved, C14a of methylene‐H 4 MPT and C4 of NADP + , between which a hydride is transferred. The two diastereotopic protons at C14a of methylene‐H 4 MPT and at C4 of NADPH can be seen separately in 1 H‐NMR spectra. This fact was used to determine the stereospecificity...