Cell-Free Protein Synthesis System Prepared from Insect Cells by Freeze-Thawing

Volume: 22, Issue: 6, Pages: 1570 - 1577
Published: Jan 1, 2006
Abstract
We established a novel cell-free protein synthesis system derived from Trichoplusia ni (HighFive) insect cells by a simple extraction method. Luciferase and beta-galactosidase were synthesized in this system with active forms. We analyzed and optimized (1) the preparation method of the insect cell extract, (2) the concentration of the reaction components, and (3) the 5'-untranslated region (5'-UTR) of mRNA. The extract was prepared by...
Paper Details
Title
Cell-Free Protein Synthesis System Prepared from Insect Cells by Freeze-Thawing
Published Date
Jan 1, 2006
Volume
22
Issue
6
Pages
1570 - 1577
Citation AnalysisPro
  • Scinapse’s Top 10 Citation Journals & Affiliations graph reveals the quality and authenticity of citations received by a paper.
  • Discover whether citations have been inflated due to self-citations, or if citations include institutional bias.