Cell-Free Protein Synthesis System Prepared from Insect Cells by Freeze-Thawing
Abstract
We established a novel cell-free protein synthesis system derived from Trichoplusia ni (HighFive) insect cells by a simple extraction method. Luciferase and beta-galactosidase were synthesized in this system with active forms. We analyzed and optimized (1) the preparation method of the insect cell extract, (2) the concentration of the reaction components, and (3) the 5'-untranslated region (5'-UTR) of mRNA. The extract was prepared by...
Paper Details
Title
Cell-Free Protein Synthesis System Prepared from Insect Cells by Freeze-Thawing
Published Date
Jan 1, 2006
Journal
Volume
22
Issue
6
Pages
1570 - 1577
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