Properties of a Novel Thermostable Glucoamylase from the Hyperthermophilic Archaeon Sulfolobus solfataricus in Relation to Starch Processing

A gene ( ssg ) encoding a putative glucoamylase in a hyperthermophilic archaeon, Sulfolobus solfataricus , was cloned and expressed in Escherichia coli , and the properties of the recombinant protein were examined in relation to the glucose production process. The recombinant glucoamylase was extremely thermostable, with an optimal temperature at 90°C. The enzyme was most active in the pH range from 5.5 to 6.0. The enzyme liberated β- d -glucose...