Purification of Lysozyme from Protein Mixtures by Solvent‐Freeze‐Out Technology

The purification of hen egg white lysozyme from a lysozyme/crude extract mixture was carried out by the solvent‐freeze‐out (SFO) technology on the laboratory scale. Compared to previous works, this investigation focuses on the crystallization of lysozyme from a complicated system. The crystallization conditions were determined by screening tests. Based on these results, the crystallization process was performed in basic buffer solution in which...