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A physical, genetic and functional sequence assembly of the barley genome

Published on Nov 29, 2012in Nature43.07
· DOI :10.1038/nature11543
Klaus F. X. Mayer69
Estimated H-index: 69
,
Robbie Waugh67
Estimated H-index: 67
(James Hutton Institute)
+ 69 AuthorsNils Stein63
Estimated H-index: 63
(Leibniz Association)
Sources
Abstract
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
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