High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos
Abstract
Recently, gene editing with transcription activator-like effector nucleases (TALENs) has been used in the life sciences. TALENs can be easily customized to recognize a specific DNA sequence and efficiently introduce double-strand breaks at the targeted genomic locus. Subsequent non-homologous end-joining repair leads to targeted gene disruption by base insertion, deletion, or both. Here, to readily evaluate the efficacy of TALENs in Xenopus...
Paper Details
Title
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos
Published Date
Mar 4, 2013
Journal
Volume
2
Issue
5
Pages
448 - 452
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