Investigation of beta-carotene gelatin composite particles with a multiwavelength UV/vis detector for the analytical ultracentrifuge

Published on Feb 1, 2010in European Biophysics Journal2.527
· DOI :10.1007/s00249-009-0412-6
Engin Karabudak9
Estimated H-index: 9
(MPG: Max Planck Society),
Wendel Wohlleben40
Estimated H-index: 40
Helmut Cölfen74
Estimated H-index: 74
(MPG: Max Planck Society)
A multiwavelength UV/vis detector for the analytical ultracentrifuge (MWL-AUC) has been developed recently. In this work, β-carotene–gelatin composite particles are investigated with MWL-AUC. Band centrifugation with a Vinograd cell is used to ensure maximum sample separation. Spectral changes of the system are observed in dependence of the sedimentation coefficient and are attributed to a previously unknown inhomogeneity of the β-carotene chemical composition with both H- and J-aggregates coexisting in a mixture. In addition, our data suggest that pure H- and J-aggregates exist in a particle while their relative concentrations in a mixture determine the color characteristics of the sample. The unique abilities and properties of MWL-AUC include sedimentation coefficient distributions for all possible wavelengths, full UV/vis spectra of each different species in the mixture and 3D movies of the sedimentation process. These properties significantly extend the scope of the analytical ultracentrifuge technique and show that complex biopolymer multicomponent mixtures can be resolved into their individual species.
  • References (12)
  • Citations (20)
📖 Papers frequently viewed together
41 Citations
2,576 Citations
29 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Holger M. Strauss (MPG: Max Planck Society)H-Index: 12
#2Engin Karabudak (MPG: Max Planck Society)H-Index: 9
Last. Helmut Cölfen (MPG: Max Planck Society)H-Index: 74
view all 6 authors...
The optical setup and the performance of a prototype UV/Vis multiwavelength analytical ultracentrifuge (MWL-AUC) is described and compared to the commercially available Optima XL-A from Beckman Coulter. Slight modifications have been made to the optical path of the MWL-AUC. With respect to wavelength accuracy and radial resolution, the new MWL-AUC is found to be comparable to the existing XL-A. Absorbance accuracy is dependent on the light intensity available at the detection wavelength as well ...
41 CitationsSource
#1Saroj Kumar Bhattacharyya (MPG: Max Planck Society)H-Index: 2
#2Patrycja Maciejewska (MPG: Max Planck Society)H-Index: 1
Last. Helmut Cölfen (MPG: Max Planck Society)H-Index: 74
view all 7 authors...
The advantages of simultaneously detecting multiple wavelengths in ultracentrifugation experiments are obvious, especially for interacting systems. In addition, the detection of the wavelength dependence of turbidity opens up the possibility to obtain independent information on the particle size in addition to the usual sedimentation coefficient distribution for colloidal systems. We therefore made an effort to develop a fast UV/Vis detector, which is able to simultaneously detec the range from ...
29 CitationsSource
#1David J. ScottH-Index: 29
#2Stephen E. HardingH-Index: 54
Last. Arthur J. RoweH-Index: 32
view all 3 authors...
158 CitationsSource
Protein interactions can promote the reversible assembly of multiprotein complexes, which have been identified as critical elements in many regulatory processes in cells. The biophysical characterization of assembly products, their number and stoichiometry, and the dynamics of their interactions in solution can be very difficult. A classical first-principle approach for the study of purified proteins and their interactions is sedimentation velocity analytical ultracentrifugation. This approach a...
100 CitationsSource
547 CitationsSource
#1Ian K. MacGregor (UNH: University of New Hampshire)H-Index: 1
#2Arthur L. Anderson (UNH: University of New Hampshire)H-Index: 4
Last. Thomas M. Laue (UNH: University of New Hampshire)H-Index: 35
view all 3 authors...
Analytical ultracentrifugation (AUC) provides first-principle hydrodynamic and thermodynamic information concerning the size, shape and interactions of macromolecules.The fundamental measurement needed in AUC is the macromolecular concentration as a function of radial position and time.Currently, the Beckman Coulter XLI analytical ultracentrifuge may be equipped with absorbance and refractive detectors, which provide complementary concentration determinations.For detecting trace quantities of ma...
96 CitationsSource
#1Helmut AuweterH-Index: 3
Last. Horst WeissH-Index: 4
view all 7 authors...
A combination of analytical methods and molecular modeling calculations has provided a detailed picture of the supramolecular and microscopic structure of precipitated lipophilic carotenoids. The nanoparticles have a core/shell structure (see schematic representation) in which the particle core (120 nm) consists of a variety of molecular aggregates of different sizes, and the shell (40 nm) consists of an adsorbed gelatin layer.
143 CitationsSource
#1Peter N. Lavrenko (RAS: Russian Academy of Sciences)H-Index: 11
#2Victor Lavrenko (UMass: University of Massachusetts Amherst)H-Index: 27
Last. V. Tsvetkov (RAS: Russian Academy of Sciences)H-Index: 1
view all 3 authors...
The polarized-light interference method is applied to visualize a distribution of macromolecule concentrations based on displacements in an ultracentrifugal cell. The optical system based on the Lebedev interferometer is presented. The sensitivity and precision of the system are compared with those of the widely used Schlieren optical system and Rayleigh interference optics when applied to synthetic polymer dilute solutions. Some questions of methodology are discussed.
12 CitationsSource
#1Stephen E. HardingH-Index: 54
#2Arthur J. RoweH-Index: 32
Last. J. C. HortonH-Index: 5
view all 3 authors...
Part I: Instrumentation Part II: Equilibrium Methods Part III: Transport Methods Part IV: Specific Applications to Biochemical and Polymer Systems
1,058 Citations
The ultracentrifugation method for determining the particle-size distribution of dispersions has been automated and its precision and capacity improved.
43 CitationsSource
Cited By20
#1Fong Kwong Yam (Universiti Sains Malaysia)
#2Joshua John Samuel (Universiti Sains Malaysia)H-Index: 1
The use of textile dyes remain relevant in the issue of water pollution. With the potential harm it may possess on the local flora and fauna, various methods of dye removal have been researched and employed, including photocatalytic degradation. TiO2, being a suitable semiconductor for this purpose needs to be sensitized prior to use, either by doping or chemical adsorption in order to activate in visible light. In this study, various natural dyes were employed to sensitize TiO2. It was found th...
#1Joseph Z. PearsonH-Index: 5
#2Helmut CölfenH-Index: 1
Open-source Multiwavelength Analytical Ultracentrifugation (MWL-AUC) detection systems have been evolving for over a decade. Continual advances emerging out of several research groups have brought the instrumentation technology to increasingly higher levels of performance. The capabilities of MWL-AUC have been documented in many publications, demonstrating the applicability of broad spectrum absorbance acquisitions in analytical ultracentrifugation to a wide array of scientific fields. Despite n...
1 CitationsSource
#1Thaseem Thajudeen (FAU: University of Erlangen-Nuremberg)H-Index: 9
#2Johannes Walter (FAU: University of Erlangen-Nuremberg)H-Index: 16
Last. Wolfgang Peukert (FAU: University of Erlangen-Nuremberg)H-Index: 47
view all 4 authors...
In this study, it is demonstrated how Brownian dynamics (BD) simulations can be used as a comprehensive forward model for analytical ultracentrifugation (AUC) and analytical centrifugation experiments. BD simulations are a versatile alternative to the numerical solution of Lamm's equation. Time dependent concentration profiles can be accurately predicted by means of the BD simulations even for colloidal systems with multiple species. It is shown that in combination with Mie's theory for light sc...
5 CitationsSource
#1Engin Karabudak (İzmir Institute of Technology)H-Index: 9
#2Emre H Brookes (University of Texas Health Science Center at San Antonio)H-Index: 17
Last. Helmut Cölfen (MPG: Max Planck Society)H-Index: 74
view all 11 authors...
1 CitationsSource
#1Engin Karabudak (İzmir Institute of Technology)H-Index: 9
#2Emre H Brookes (University of Texas Health Science Center at San Antonio)H-Index: 17
Last. Helmut Cölfen (MPG: Max Planck Society)H-Index: 74
view all 11 authors...
We report an unsurpassed solution characterization technique based on analytical ultracentrifugation, which demonstrates exceptional potential for resolving particle sizes in solution with sub-nm resolution. We achieve this improvement in resolution by simultaneously measuring UV/Vis spectra while hydrodynamically separating individual components in the mixture. By equipping an analytical ultracentrifuge with a novel multi-wavelength detector, we are adding a new spectral discovery dimension to ...
12 CitationsSource
#1Abhiksha Desai (NIH: National Institutes of Health)H-Index: 1
#2Jonathan Krynitsky (CIT: Center for Information Technology)H-Index: 3
Last. Peter Schuck (NIH: National Institutes of Health)H-Index: 64
view all 5 authors...
Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution. A key mechanical element in AUC is the centerpiece, a component of the sample cell assembly that is mounted between the optical windows to allow imaging and to seal the sample solution column against high vacuum while exposed to gravitational forces in exce...
8 CitationsSource
#1Johannes Walter (FAU: University of Erlangen-Nuremberg)H-Index: 16
#2Doris Segets (FAU: University of Erlangen-Nuremberg)H-Index: 19
Last. Wolfgang Peukert (FAU: University of Erlangen-Nuremberg)H-Index: 47
view all 3 authors...
5 CitationsSource
#1Engin Karabudak (İzmir Institute of Technology)H-Index: 2
#2Helmut Cölfen (University of Konstanz)H-Index: 74
The multiwavelength (MWL) detector is a new type of absorption detector for AUC. The commercial absorption detector of the Beckman Coulter XL-A AUC can only handle a single wavelength per scan with the possibility to scan at maximum 3 wavelengths, whereas MWL-AUC can handle all the wavelengths in the UV/Vis region at one time. The result is impressive since now a full spectral dimension is added to each single scan. In this chapter, we are explaining development history, instrumentation, and fut...
2 CitationsSource
#1Hideki Hashimoto (Kwansei Gakuin University)H-Index: 32
#2Yuko Sugai (Kwansei Gakuin University)H-Index: 3
Last. Richard J. Cogdell (Glas.: University of Glasgow)H-Index: 61
view all 5 authors...
Carotenoids are essential pigments in natural photosynthesis. They absorb in the blue–green region of the solar spectrum and transfer the absorbed energy to (bacterio-)chlorophylls, and so expand the wavelength range of light that is able to drive photosynthesis. This process is an example of singlet–singlet energy transfer and so carotenoids serve to enhance the overall efficiency of photosynthetic light reactions. Carotenoids also act to protect photosynthetic organisms from the harmful effect...
25 CitationsSource
#1Shohei Kita (OCU: Osaka City University)H-Index: 1
#2Ritsuko Fujii (OCU: Osaka City University)H-Index: 26
Last. Hideki Hashimoto (OCU: Osaka City University)H-Index: 32
view all 4 authors...
Fucoxanthin shows a large bathochromic shift of its absorption band when it is bound to photosynthetic antenna pigment–protein complexes derived in brown algae (fucoxanthin–chlorophyll a/c binding protein; FCP). In order to try to understand the intermolecular interactions of fucoxanthin responsible for this shift, we attempted to create a system that can control intermolecular interactions of carotenoids. In this study, we prepared the fucoxanthin–silica gel (CARiACT Q-6, mesoporous material) c...
7 CitationsSource