Development and evaluation of a multiplex PCR for eight plasmid-mediated quinolone-resistance determinants

The objective of this study was to develop and validate an expanded multiplex PCR assay for the simultaneous detection of eight plasmid-mediated quinolone-resistance determinants in Enterobacteriaceae . Primers were designed to amplify conserved fragments of qnrABCDS , qepA , oqxAB and aac(6′)-Ib-cr genes and were optimized in uniplex and multiplex PCR assays with control template DNA. The assay was used to determine the prevalence of...