Original paper

Determination of the Phase Diagram for Soluble and Membrane Proteins

Volume: 114, Issue: 13, Pages: 4432 - 4441
Published: Mar 17, 2010
Abstract
Methods to efficiently determine the phase behavior of novel proteins have the potential to significantly benefit structural biology efforts. Here, we present protocols to determine both the solubility boundary and the supersolubility boundary for protein/precipitant systems using an evaporation-based crystallization platform. This strategy takes advantage of the well-defined rates of evaporation that occur in this platform to determine the...
Paper Details
Title
Determination of the Phase Diagram for Soluble and Membrane Proteins
Published Date
Mar 17, 2010
Volume
114
Issue
13
Pages
4432 - 4441
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