Generation of a mouse mutant by oligonucleotide-mediated gene modification in ES cells
Abstract
Oligonucleotide-mediated gene targeting is emerging as a powerful tool for the introduction of subtle gene modifications in mouse embryonic stem (ES) cells and the generation of mutant mice. However, its efficacy is strongly suppressed by DNA mismatch repair (MMR). Here we report a simple and rapid procedure for the generation of mouse mutants using transient down regulation of the central MMR protein MSH2 by RNA interference. We demonstrate...
Paper Details
Title
Generation of a mouse mutant by oligonucleotide-mediated gene modification in ES cells
Published Date
Nov 16, 2006
Journal
Volume
34
Issue
21
Pages
e147 - e147
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