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Comparison of the cytotoxic impact of chlorfluazuron on selected insect and human cell lines

Published on Jul 1, 2015in Environmental Toxicology and Chemistry3.42
· DOI :10.1002/etc.2969
Qingchun Huang8
Estimated H-index: 8
,
Xiwei Wu3
Estimated H-index: 3
+ 3 AuthorsLiming Tao7
Estimated H-index: 7
Abstract
To gain new insight into the mechanism of selective cytotoxicity of benzoylureas as insecticides, the in vitro mode of action of chlorfluazuron was investigated on lepidopteran Tn5B1-4 and Sf-21 cells and human Hek293 and HepG2 cells. Chlorfluazuron inhibited the proliferation of Tn5B1-4 and Sf-21 cells with 50% inhibitory concentration values (IC50) of 4.96 µM and 1.12 µM at 48 h and 2.37 µM and 1.76 µM at 96 h, respectively, versus that of Hek293 and HepG2 cells with IC50 values >20 µM. When transferred to chlorfluazuron-free medium, lepidopteran Tn5B1-4 and Sf-21 cells had a postinhibitory recovery development period within 24 h followed by a suppressed increase in cell viability, but human Hek293 and HepG2 cells showed an accelerated increase over their control level. Chlorfluazuron affected Tn5B1-4 and Sf-21 cells, with ≥1.8-fold decreases in the ratio of cellular N-acetylglucosamine (GlcNAc) level and protein content and ≥1.5-fold increases in the mitotic index and G2/M-phase arrest. Neither Hek293 nor HepG2 cells contained GlcNAc, and chlorfluazuron had no significant effects on the cell cycle and mitotic index of Hek293 and HepG2 cells. In conclusion, the differences between human and lepidopteran cell lines in the characteristic GlcNAc content, G2/M arrest in the cycle progress, and mitotic index of cells in response to chlorfluazuron may contribute to the selective toxicity of chlorfluazuron to lepidopteran cells. Environ Toxicol Chem 2015;34:1675–1682. © 2015 SETAC
  • References (29)
  • Citations (5)
References29
Newest
#1Entsar I. Rabea (Alexandria University)H-Index: 14
#2Hoda M. Nasr (Alexandria University)H-Index: 7
Last.Mei Badawy (Alexandria University)H-Index: 18
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#1Michael B. Ujiki (NU: Northwestern University)H-Index: 30
#2Xian Zhong Ding (NU: Northwestern University)H-Index: 9
Last.Thomas E. Adrian (NU: Northwestern University)H-Index: 57
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#1Zsuzsanna Rozmer (PTE: University of Pécs)H-Index: 6
#2Timea Berki (PTE: University of Pécs)H-Index: 23
Last.Pál Perjési (PTE: University of Pécs)H-Index: 19
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#1Sara Sousa (IPN: Instituto Politécnico Nacional)H-Index: 3
#2M. L. Maia (IPN: Instituto Politécnico Nacional)
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#1Xianghu Zhao (ECUST: East China University of Science and Technology)
#2Chunmei Li (ECUST: East China University of Science and Technology)H-Index: 1
Last.Song Cao (ECUST: East China University of Science and Technology)
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#1Solange Muhayimana (ECUST: East China University of Science and Technology)H-Index: 1
#2Xianfei Zhang (ECUST: East China University of Science and Technology)H-Index: 1
Last.Qingchun Huang (ECUST: East China University of Science and Technology)H-Index: 8
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#1Irum Shaheen (Fatima Jinnah Women University)H-Index: 1
#2Khuram Shahzad Ahmad (Fatima Jinnah Women University)H-Index: 7
Last.Taghazal Zahra (Fatima Jinnah Women University)H-Index: 1
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#1Xinming Yun (ECUST: East China University of Science and Technology)H-Index: 4
#2Qingchun Huang (ECUST: East China University of Science and Technology)H-Index: 8
Last.Ziyi Wan (ECUST: East China University of Science and Technology)H-Index: 1
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