Dynamic-Light-Scattering-Based Sequence-Specific Recognition of Double-Stranded DNA with Oligonucleotide-Functionalized Gold Nanoparticles

An ultrasensitive and simple dynamic‐light‐scattering (DLS) assay for the sequence‐specific recognition of double‐stranded DNA (dsDNA) was developed based on detection of the average diameter change of Au nanoparticle (AuNP) probes modified with oligonucleotides 5′‐TTTCTCTTCCTT‐ CTCTTC‐(T) 12 ‐SH‐3′ (Oligo 1) and 5′‐TTCTTTCTTTTCTTTTTC‐(T) 12 ‐ SH‐3′ (Oligo 2). The target dsDNA was composed of two complementary oligonucleotides:...