Crystallization of Xylanase fromErwinia chrysanthemi: Influence of Heat and Polymeric Substrate

Xylanase from the bacterial plant pathogen Erwinia chrysanthemi (E.C. 3.2.1.8), expressed in E. coli, has been crystallized for X-ray diffraction analysis both in the presence and the absence of its polymeric substrate 4-O-methyl glucuronoxylan. In all cases it was found that the quality, time of appearance, and reproducibility of both the native and complex crystals were significantly enhanced by heating of the protein to 323 K prior to...