De novo assembly and analysis of RNA-seq data
Abstract
We describe Trans-ABySS, a de novo short-read transcriptome assembly and analysis pipeline that addresses variation in local read densities by assembling read substrings with varying stringencies and then merging the resulting contigs before analysis. Analyzing 7.4 gigabases of 50-base-pair paired-end Illumina reads from an adult mouse liver poly(A) RNA library, we identified known, new and alternative structures in expressed transcripts, and...
Paper Details
Title
De novo assembly and analysis of RNA-seq data
Published Date
Oct 10, 2010
Journal
Volume
7
Issue
11
Pages
909 - 912
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