Direct demonstration that the vitamin k‐dependent bone gla protein is incompletely γ‐carboxylated in humans

Incomplete vitamin K-dependent gamma-carboxylation has been found in bone Gla protein (BGP) isolated from each of 20 different human bone samples. Using N-terminal protein sequencing of the methyl-esterified protein (Anal Biochem 1991;199:93-97), a method that directly measures the percentage of gamma-carboxylation at each target glutamate residue, the extent of incomplete BGP gamma-carboxylation was found to depend strongly on sequence...