ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Abstract
Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a nonspecific DNA cleavage domain. ZFNs and TALENs enable a broad range of genetic modifications by inducing DNA double-strand breaks that stimulate error-prone...
Paper Details
Title
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Published Date
Jul 1, 2013
Journal
Volume
31
Issue
7
Pages
397 - 405
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