Improving protein crystal quality by decoupling nucleation and growth in vapor diffusion
Abstract
A simple method for growing protein crystals in the metastable zone using the vapor diffusion technique is described. The coverslips holding the hanging drops are transferred, after being incubated for some time at conditions normally giving many small crystals, over reservoirs at concentrations that normally yield clear drops. Fewer, much larger and better diffracting crystals are obtained, compared with conventional crystallization at similar...
Paper Details
Title
Improving protein crystal quality by decoupling nucleation and growth in vapor diffusion
Published Date
Jan 1, 2000
Journal
Volume
9
Issue
4
Pages
755 - 757
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