Differential Bacterial Surface Display of Peptides by the Transmembrane Domain of OmpA
Abstract
Peptide libraries or antigenic determinants can be displayed on the surface of bacteria through insertion in a suitable outer membrane scaffold protein. Here, we inserted the well-known antibody epitopes 3xFLAG and 2xmyc in exterior loops of the transmembrane (TM) domain of OmpA. Although these highly charged epitopes were successfully displayed on the cell surface, their levels were 10-fold reduced due to degradation. We verified that the...
Paper Details
Title
Differential Bacterial Surface Display of Peptides by the Transmembrane Domain of OmpA
Published Date
Aug 25, 2009
Journal
Volume
4
Issue
8
Pages
e6739 - e6739
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