Time-Controlled Microfluidic Seeding in nL-Volume Droplets To Separate Nucleation and Growth Stages of Protein Crystallization
Abstract
Sowing seeds: The growth of well-ordered protein crystals requires control of the two key stages of crystallization, namely, nucleation and growth. However, the ideal conditions for these two stages are often different. A microfluidic system (see picture) was used to perform time-controlled crystal seeding in nL volumes. Single crystals of the protein Oligoendopeptidase F were obtained and used to determine its X-ray crystal structure....
Paper Details
Title
Time-Controlled Microfluidic Seeding in nL-Volume Droplets To Separate Nucleation and Growth Stages of Protein Crystallization
Published Date
Dec 4, 2006
Journal
Volume
45
Issue
48
Pages
8156 - 8160
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