Modulation of the clastogenic activity of gamma-irradiation in buthionine sulphoximine-mediated glutathione depleted mammalian cells

Chromosome aberrations (CA) were used as an end-point to investigate the effect of buthionine sulphoximine (BSO), a potent glutathione-depleting agent, on the radiosensitivity of mammalian cells. The aim was to obtain information about the role of glutathione (GSH) in physicochemical and biochemical processes in irradiated cells.CA were scored from first cycle metaphases in irradiated BSO-pretreated and untreated samples. BSO exposure was for...